FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2232_d06_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2232_d06_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1816124
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA217891.1997528803099349No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCC191171.0526263625170968No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG182971.0074752604998338No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG151150.8322669597450394No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT109860.6049146423922596No Hit
ATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACACCATG97500.5368576154491653No Hit
CTTCAATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTA90120.4962216236336286No Hit
CACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGAACG87920.4841079133363141No Hit
TTCTTAATCCTTCCAGACTCGAAGTCAATCCGTGCATCAATTCGGGCCCT87360.48102442344245216No Hit
TAATTAAGAAGTACACATCAGGAAGACAGGAGAAGAATCCCGCTCTTAGA82260.4529426404804958No Hit
ATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAA79360.43697456781585403No Hit
CTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCA72390.3985961311011803No Hit
CTACTACTCATTGTTCAAGCACTTAGGGACAACCTGGAACCTGGAACCTT66590.3666599857718966No Hit
ATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTG64860.35713420449264477No Hit
GGATACACCATGGACACAGTCAACAGAACACATCAATATTCAGAAAAAGG62530.3443046840413981No Hit
TTTCTTAAAAGTGCCAGCGCAAAATGCAATAAGTACCACATTCCCTTATA61070.3362655853895439No Hit
CATCAATATTCAGAAAAAGGGAGGTGGACAACAAAACACTAGCCAAAGGG56090.3088445502619865No Hit
AAGTACCACATTCCCTTATACTGGAGATCCCCCATATAGCCATGGAACAG54270.2988232081069354No Hit
GATCTTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTTCCAGACTCGA52830.2908942340941478No Hit
GCCTATATTCATCTCCACAACTCGAAGGATTTTCAGCTGAATCGAGAAAA52690.2901233616206823No Hit
TTCCAGACTCGAAGTCAATCCGTGCATCAATTCGGGCCCTAGACACCATG51190.28186401369069514No Hit
GCCAAATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATC49190.2708515497840456No Hit
CTATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCA44910.24728487702381555No Hit
ATGGAAGACTTTGTGCGACAGTGCTTCAATCCAATGATTGTCGAGCTTGC43440.23919071605242814No Hit
CCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAG43420.23908059141336163No Hit
TCTTTCTTAATCCTTCCAGACTCGAAGTCAATCCGTGCATCAATTCGGGC42640.2347857304897683No Hit
CCATATAGCCATGGAACAGGAACAGGATACACCATGGACACAGTCAACAG41890.23065605652477475No Hit
AAGTCAATCCGTGCATCAATTCGGGCCCTAGACACCATGGCCTCCATCAT40600.22355301730498578No Hit
TTTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTA40370.22228658395572107No Hit
CATCAATACCAGAAGTGCTGCACTCTATTCGAGAAATTCTTCCCTAGCAG40230.2215157114822556No Hit
GTACCACATTCCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGA39480.21738603751726204No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTCC38200.2103380606170063No Hit
GCTAGGGAAGAATTTCTCGAATAGAGTGCAGCACTTCTGGTACATTTGTT38070.20962225046307412No Hit
CTACTAACCTGGAACCTGGAACCTTTGATCTTGAAGGGCTATATGGAGCA37880.2085760663919424No Hit
GCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGAAGGAG37580.20692419680594498No Hit
GTTATATAGATTGTTCACGTTCGCATCATGCTCATCGAGTGTTTTTTGAT37010.20378564459254986No Hit
GGATACACCATGGACACAGTCAACAGAACACATCAATACCAGAAGTGCTG35790.19706804160949362No Hit
CCCCCATATAGCCATGGAACAGGAACAGGATACACCATGGACACAGTCAA35620.19613198217742842No Hit
GTCCCTAAGTGCTTGAACAATGAGTAGTAGTTTTCTCGATTCAGCTGAAA35520.19558135898209592No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCTTGCCG35470.1953060473844297No Hit
CCATTTGAATGGATGTCAATCCGACTTTACTTTTCTTAAAAGTGCCAGCG35160.19359911547889902No Hit
TTGTGCGACAGTGCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACA34860.19194724589290157No Hit
CATCAATATTCAGAAAAAGGGAGGTGGACAACAAACACAGAGACCGGAGC33190.18275183853084923No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG32800.18060440806905256No Hit
AGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGT32640.17972341095652059No Hit
CAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTG32060.17652979642359223No Hit
ACAAACACAGAGACCGGAGCATGCACAAACAGATTGTGTATTGGAAGCAA31960.17597917322825976No Hit
ATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATATGGGGAA31350.17262037173673164No Hit
GAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCGAGATA31260.1721248108609324No Hit
AATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATGTCGCA30770.16942675720380326No Hit
TAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGAAGGAGTTGAA30680.16893119632800405No Hit
CCATCATGCTGGAAATTCCAACTGGCCTCCGATATGAACTGCTAGGGAAG30600.16849069777173806No Hit
GCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCT30090.1656825194755424No Hit
CTAGACACCATGGCCTCCATCATGCTGGAAATTCCAACTGGCCTCCGATA29990.16513189628020994No Hit
GATATGAACTGCTAGGGAAGAATTTCTCGAATAGAGTGCAGCACTTCTGG29790.164030649889545No Hit
TCATTAATCAGGCACTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAA29610.16303952813794653No Hit
CTTCTTTCTTAATCCTTCCAGACTCGAAGTCAATCCGTGCATCAATTCGG29520.16254396726214732No Hit
GGTTAGTAGTTTTCTCGATTCAGCTGAAAATCCTTCGAGTTGTGGAGATG29240.16100222231521635No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAT29180.16067184839801688No Hit
CTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGAACGAG29020.15979085128548493No Hit
GCTACATTTGTTCATCCTCAAGAATTCCCCTTTGGCTAGTGTTGAGAATG28870.1589649164924862No Hit
CTGCTAGGGAAGAATTTCTCGAATAGAGTGCAGCACTTCTGGTACATTTG28760.15835923097762047No Hit
CTTAGGGACAACCTGGAACCTGGAACCTTTGATCTTGAAGGGCTATATGG28690.15797379474088774No Hit
TCTTAATCCTTCCAGACTCGAAGTCAATCCGTGCATCAATTCGGGCCCTA28660.157808607782288No Hit
GTATAGGTGGGTGGGTGATGTATGCCCCACACGAAAAGAATGCTCTTTCC28650.15775354546275475No Hit
GGTTGTCCCTAAGTGCTTGAACAATGAGTAGTAGTTTTCTCGATTCAGCT28530.15709279762835576No Hit
ACAAAACACTAGCCAAAGGGGAATTCTTGAGGATGAACAAATGTACCAGA28200.1552757410837586No Hit
CCATGGACACAGTCAACAGAACACATCAATATTCAGAAAAAGGGAGGTGG28140.1549453671665591No Hit
GATCTTAAGATCTCAGCAAATTCTTCTTTCTTAATCCTTCCAGACTCGAA27970.1540093077344939No Hit
CCCATATAGCCATGGAACAGGAACAGGATACACCATGGACACAGTCAACA27200.14976950913043383No Hit
GGACAAGCCAAATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAA26960.14844801346163589No Hit
AAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTTTACTTTTCTTA26160.14404302789897608No Hit
ATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCTGCAATATGCA26010.14321709310597736No Hit
CCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGG25310.13936273073865002No Hit
CTCTTCAATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCT25190.13870198290425104No Hit
ATCAATACATTAGCTTTCTCCCCTTTCGTAAGATTGCTCAGTTCATTGAT25120.13831654666751828No Hit
GGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATG24870.13693998867918708No Hit
GCTTCATGTATTCAGACTTCCATTTCATTGACGAACGAGGCGAATCAATA24860.13688492635965385No Hit
GTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTTCC24480.13479255821739045No Hit
CTTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTTCCAGACTCGAAGT24340.13402168574392498No Hit
ATCAGGCACTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCC24220.13336093790952602No Hit
GCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATAT24180.133140688631393No Hit
GGCCCTAGACACCATGGCCTCCATCATGCTGGAAATTCCAACTGGCCTCC24050.1324248784774608No Hit
ATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTAATGCATCTTG23520.12950657554219866No Hit
GTTCCAGGTTGTCCCTAAGTGCTTGAACAATGAGTAGTAGTTTTCTCGAT23270.12813001755386746No Hit
GAATAATATAGTCGACAAGATGAACAAGCAATATGAAATAATTGATCATG23270.12813001755386746No Hit
CCATGGCCTCCATCATGCTGGAAATTCCAACTGGCCTCCGATATGAACTG23190.12768951899760148No Hit
GTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCTT23180.12763445667806822No Hit
CACCTATACCGAGCAAACAAATTTGTACATAAGAAACGACACAACAACAA23170.127579394358535No Hit
CCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATCTTCAAGATCTCAG23110.1272490204413355No Hit
TGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTT23070.12702877116320252No Hit
ATGAAGGACAAGCCAAATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGG23030.12680852188506953No Hit
GTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCCAATGA22920.1262028363702038No Hit
TAATTACACATCAGGAAGACAGGAGAAGAATCCCGCTCTTAGAATGAAAT22870.12592752477253755No Hit
ACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTCCATATAGCCCTTC22590.12438577982560661No Hit
GAACAGGAACAGGATACACCATGGACACAGTCAACAGAACACATCAATAT22530.12405540590840715No Hit
GAGTTGAACCAAGATGCATTAAGCAAAACCCAGGGATCATTAATCAGGCA21960.12091685369501201No Hit
CTCGAAGTCAATCCGTGCATCAATTCGGGCCCTAGACACCATGGCCTCCA21930.12075166673641227No Hit
GGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGA21800.12003585658248006No Hit
ATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATCTTCAA21650.11920992178948134No Hit
GCCCTAGACACCATGGCCTCCATCATGCTGGAAATTCCAACTGGCCTCCG21550.11865929859414887No Hit
GTCCTACATCGTCGAAAGATCATCAGCTGTAAATGGAACGTGTTACCCTG21520.11849411163554911No Hit
GGCCTCCATCATGCTGGAAATTCCAACTGGCCTCCGATATGAACTGCTAG21290.11722767828628441No Hit
GTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATT21070.11601630725655296No Hit
GTTCATTGATGCTTAATGCTGGTCCATATCTTTTGTCTTCTTTGCCGAGG21020.11574099565888672No Hit
GAATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCTGCAATATG20960.11541062174168724No Hit
GGTTAGTAGTTTTCTCGATTCAGCTGAAAATCCTTCGAGTTGAAGCAGAC20710.11403406375335605No Hit
ATCTTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTTCCAGACTCGAA20620.11353850287755682No Hit
ATTCGGGCCCTAGACACCATGGCCTCCATCATGCTGGAAATTCCAACTGG20480.11276763040409135No Hit
ATGCAATAAGTACCACATTCCCTTATACTGGAGATCCCCCATATAGCCAT20400.11232713184782536No Hit
TTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTTCCAGACTCGAAGTC20240.11144613473529341No Hit
TTCTTCTTTCTTAATCCTTCCAGACTCGAAGTCAATCCGTGCATCAATTC20190.11117082313762718No Hit
TCTTAAAAGTGCCAGCGCAAAATGCAATAAGTACCACATTCCCTTATACT20040.11034488834462845No Hit
GTGTTGAGAATGGAGCGATTTCTCTTGGGAATCCATGAATGTGTGGGATT19760.10880314339769752No Hit
ACAGTGCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGG19720.10858289411956451No Hit
ATATTATTCACCTTGGATGTTATTTTATCAATTGCCTTTTGAGTTGAATC19710.10852783180003128No Hit
CTTCTAAGTGTGTGCATATTGCAGCGAACTTATTTGTTTCAATTTTCGGG19650.10819745788283178No Hit
CTTAAGATCTCAGCAAATTCTTCTTTCTTAATCCTTCCAGACTCGAAGTC19490.10731646077029983No Hit
GATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCCAATGATTGTC19320.10638040133823462No Hit
GTGCGACAGTGCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAAT19290.10621521437963488No Hit
GCTTAATGCTGGTCCATATCTTTTGTCTTCTTTGCCGAGGATTAGAAATC19260.10605002742103513No Hit
ACCATGGACACAGTCAACAGAACACATCAATATTCAGAAAAAGGGAGGTG18910.10412284623737146No Hit
ATGTCTGGCCTGGTTTTAGTACCGACCAATAATAATCAATTCTTCCCTGC18900.1040677839178382No Hit
ACCTGGAACCTGGAACCTTTGATCTTGAAGGGCTATATGGAGCAATTGAG18780.10340703608343924No Hit
GTTCATATCGGAGGCCAGTTGGAATTTCCAGCATGATGGAGGCCATGGTG18660.10274628824904027No Hit
CTATTCGAGAAATTCTTCCCTAGCAGTTCATATCGGAGGCCAGTTGGAAT18370.10114948098257609No Hit
GCAATATGCACACACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTT18320.10087416938490984No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGCCG151.2296523E-4145.01533
GCAGGTA25100.0121.038672
CAGGTAC26650.0113.726823
GGTACTG26750.0113.301685
CGCCGTC203.8649474E-4108.761485
GTACTGA28650.0105.5347066
AGTAGAA23250.0100.7310641
TAGCGCG950.099.210075145
GGTCAAA5350.093.514551
ACTGATC39050.086.145588
AGCGAAA3200.086.102841
AGGTACT36600.085.1865844
AGCAGGT36400.083.6626741
AAATATA6300.079.413145
GCGAAAG3550.077.613822
TAGAAAC30550.076.898463
AGAAACA32400.072.7314454
CGCAATT406.664802E-572.499664145
TACTGAT43100.071.153977
ACAAGGC23100.070.616568
Produced by FastQC (version 0.11.5)