FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2232_d06_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2232_d06_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1284170
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA144481.1250846850494873No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG134091.044176394091125No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCC129291.0067981653519393No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG124790.971756075908953No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT88570.6897061915478481No Hit
ATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACACCATG70360.5479025362685626No Hit
TAATTAAGAAGTACACATCAGGAAGACAGGAGAAGAATCCCGCTCTTAGA63910.4976755414002819No Hit
CACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGAACG63380.4935483619769968No Hit
TTCTTAATCCTTCCAGACTCGAAGTCAATCCGTGCATCAATTCGGGCCCT60280.4694082559162728No Hit
ATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAA60260.4692525132965262No Hit
CTTCAATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTA53980.42034933069609165No Hit
ATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTG46330.3607777786430145No Hit
CTACTACTCATTGTTCAAGCACTTAGGGACAACCTGGAACCTGGAACCTT45220.3521340632470779No Hit
CTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCA40880.31833791476206424No Hit
GGATACACCATGGACACAGTCAACAGAACACATCAATATTCAGAAAAAGG40370.31436647795852574No Hit
AAGTACCACATTCCCTTATACTGGAGATCCCCCATATAGCCATGGAACAG40220.31319840831042617No Hit
CATCAATATTCAGAAAAAGGGAGGTGGACAACAAAACACTAGCCAAAGGG40000.3114852394932135No Hit
GCCTATATTCATCTCCACAACTCGAAGGATTTTCAGCTGAATCGAGAAAA38360.29871434467399177No Hit
TTTCTTAAAAGTGCCAGCGCAAAATGCAATAAGTACCACATTCCCTTATA37950.2955216209691863No Hit
GATCTTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTTCCAGACTCGA33900.2639837404704985No Hit
TTCCAGACTCGAAGTCAATCCGTGCATCAATTCGGGCCCTAGACACCATG33290.2592335905682269No Hit
TCTTTCTTAATCCTTCCAGACTCGAAGTCAATCCGTGCATCAATTCGGGC33200.2585327487793672No Hit
CCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAG31640.2463848244391319No Hit
GCCAAATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATC31590.24599546788976537No Hit
ATGGAAGACTTTGTGCGACAGTGCTTCAATCCAATGATTGTCGAGCTTGC31490.24521675479103233No Hit
CTATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCA31310.2438150712133129No Hit
TTTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTA29600.230499077224978No Hit
CCATATAGCCATGGAACAGGAACAGGATACACCATGGACACAGTCAACAG29240.2276957100695391No Hit
CTACTAACCTGGAACCTGGAACCTTTGATCTTGAAGGGCTATATGGAGCA28890.22497021422397345No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAT28750.22388001588574724No Hit
GCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGAAGGAG28410.2212323913500549No Hit
GCTAGGGAAGAATTTCTCGAATAGAGTGCAGCACTTCTGGTACATTTGTT27910.21733882585638972No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCTTGCCG27470.21391248822196438No Hit
GTACCACATTCCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGA26970.2100189227282992No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTCC26850.20908446700981959No Hit
AAGTCAATCCGTGCATCAATTCGGGCCCTAGACACCATGGCCTCCATCAT26510.20643684247412725No Hit
CCATTTGAATGGATGTCAATCCGACTTTACTTTTCTTAAAAGTGCCAGCG26470.20612535723463404No Hit
GTCCCTAAGTGCTTGAACAATGAGTAGTAGTTTTCTCGATTCAGCTGAAA26350.2051909015161544No Hit
AGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGT25540.19888332541641682No Hit
GAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCGAGATA25530.19880545410654354No Hit
GATATGAACTGCTAGGGAAGAATTTCTCGAATAGAGTGCAGCACTTCTGG25280.19685867135971094No Hit
CATCAATACCAGAAGTGCTGCACTCTATTCGAGAAATTCTTCCCTAGCAG25190.19615782957085123No Hit
CTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGAACGAG24960.19436678944376523No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG24330.1894608969217471No Hit
ATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATATGGGGAA23940.1864239158366883No Hit
AAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTTTACTTTTCTTA23730.1847886183293489No Hit
TTGTGCGACAGTGCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACA23670.1843213904701091No Hit
GGTTAGTAGTTTTCTCGATTCAGCTGAAAATCCTTCGAGTTGTGGAGATG23570.18354267737137606No Hit
ATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCTGCAATATGCA23530.18323119213188285No Hit
CCCCCATATAGCCATGGAACAGGAACAGGATACACCATGGACACAGTCAA23490.18291970689238965No Hit
GGTTGTCCCTAAGTGCTTGAACAATGAGTAGTAGTTTTCTCGATTCAGCT23210.18073931021593714No Hit
CAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTG23070.1796491118777109No Hit
CTTCTTTCTTAATCCTTCCAGACTCGAAGTCAATCCGTGCATCAATTCGG22810.177624457821005No Hit
TAATTACACATCAGGAAGACAGGAGAAGAATCCCGCTCTTAGAATGAAAT22800.1775465865111317No Hit
TAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGAAGGAGTTGAA22760.1772351012716385No Hit
CATCAATATTCAGAAAAAGGGAGGTGGACAACAAACACAGAGACCGGAGC22620.17614490293341223No Hit
ATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTAATGCATCTTG22260.17334153577797332No Hit
TCTTAATCCTTCCAGACTCGAAGTCAATCCGTGCATCAATTCGGGCCCTA22220.1730300505384801No Hit
ATCAATACATTAGCTTTCTCCCCTTTCGTAAGATTGCTCAGTTCATTGAT22200.1728743079187335No Hit
TCATTAATCAGGCACTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAA21980.17116113910152084No Hit
GGATACACCATGGACACAGTCAACAGAACACATCAATACCAGAAGTGCTG21970.1710832677916475No Hit
CTGCTAGGGAAGAATTTCTCGAATAGAGTGCAGCACTTCTGGTACATTTG21690.16890287111519503No Hit
ACAAACACAGAGACCGGAGCATGCACAAACAGATTGTGTATTGGAAGCAA21460.16711183098810906No Hit
CTTAGGGACAACCTGGAACCTGGAACCTTTGATCTTGAAGGGCTATATGG20500.15963618524027193No Hit
ACAAAACACTAGCCAAAGGGGAATTCTTGAGGATGAACAAATGTACCAGA19900.1549639066478737No Hit
GCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCT19850.1545745500985072No Hit
GTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATT19820.1543409361688873No Hit
CCATGGACACAGTCAACAGAACACATCAATATTCAGAAAAAGGGAGGTGG19630.15286138128129453No Hit
ATGAAGGACAAGCCAAATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGG19560.1523162821121814No Hit
ATATGGAGCAATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTA19340.15060311329496873No Hit
CCATCATGCTGGAAATTCCAACTGGCCTCCGATATGAACTGCTAGGGAAG19230.1497465288863624No Hit
GCTTCATGTATTCAGACTTCCATTTCATTGACGAACGAGGCGAATCAATA19160.14920142971724928No Hit
GTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCTT19130.14896781578762935No Hit
GGACAAGCCAAATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAA19050.14834484530864292No Hit
CCCATATAGCCATGGAACAGGAACAGGATACACCATGGACACAGTCAACA18990.14787761744940311No Hit
GCTACATTTGTTCATCCTCAAGAATTCCCCTTTGGCTAGTGTTGAGAATG18390.14320533885700493No Hit
CCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGG18250.14211514051877866No Hit
GAATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCTGCAATATG18150.14133642742004562No Hit
GAAAAGACAATGAAGGAATATGGGGAAGACCCGAAAATTGAAACAAATAA17360.13518459394005466No Hit
GCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATAT17190.1338607816722085No Hit
GTTCCAGGTTGTCCCTAAGTGCTTGAACAATGAGTAGTAGTTTTCTCGAT17190.1338607816722085No Hit
CTAGACACCATGGCCTCCATCATGCTGGAAATTCCAACTGGCCTCCGATA17180.13378291036233522No Hit
CTTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTTCCAGACTCGAAGT17150.1335492964327153No Hit
TCTTAAAAGTGCCAGCGCAAAATGCAATAAGTACCACATTCCCTTATACT16640.1295778596291768No Hit
GTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCCAATGA16630.1294999883193035No Hit
GAGTAGTAGTTTTCTCGATTCAGCTGAAAATCCTTCGAGTTGTGGAGATG16600.1292663743896836No Hit
GGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGA16590.1291885030798103No Hit
CCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATCTTCAAGATCTCAG16330.12716384902310443No Hit
CTCTTCAATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCT15890.12373751138867906No Hit
GCAATATGCACACACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTT15870.12358176876893247No Hit
ATCAGGCACTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCC15760.12272518436032612No Hit
GGAATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCTGCAATAT15690.122180085191213No Hit
ATATTCATCTCCACAACTCGAAGGATTTTCAGCTGAATCGAGAAAACTAC15670.1220243425714664No Hit
ATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATCTTCAA15650.1218685999517198No Hit
CTTCTAAGTGTGTGCATATTGCAGCGAACTTATTTGTTTCAATTTTCGGG15390.1198439458950139No Hit
GTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTTCC15290.11906523279628088No Hit
GGCCCTAGACACCATGGCCTCCATCATGCTGGAAATTCCAACTGGCCTCC15150.11797503445805461No Hit
TGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTT14730.11470443944337587No Hit
ACCTGGAACCTGGAACCTTTGATCTTGAAGGGCTATATGGAGCAATTGAG14670.11423721158413606No Hit
GAGTTGAACCAAGATGCATTAAGCAAAACCCAGGGATCATTAATCAGGCA14600.11369211241502293No Hit
CCATGGCCTCCATCATGCTGGAAATTCCAACTGGCCTCCGATATGAACTG14430.11236830014717678No Hit
GATCTTAAGATCTCAGCAAATTCTTCTTTCTTAATCCTTCCAGACTCGAA14390.11205681490768357No Hit
ATGCAATAAGTACCACATTCCCTTATACTGGAGATCCCCCATATAGCCAT14380.11197894359781026No Hit
ACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTCCATATAGCCCTTC14360.11182320097806367No Hit
TTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTTCCAGACTCGAAGTC14310.11143384442869715No Hit
CTCGAAGTCAATCCGTGCATCAATTCGGGCCCTAGACACCATGGCCTCCA14310.11143384442869715No Hit
GTATAGGTGGGTGGGTGATGTATGCCCCACACGAAAAGAATGCTCTTTCC14180.11042151740034419No Hit
GTTCATATCGGAGGCCAGTTGGAATTTCCAGCATGATGGAGGCCATGGTG14140.11011003216085098No Hit
GTTCATTGATGCTTAATGCTGGTCCATATCTTTTGTCTTCTTTGCCGAGG14100.10979854692135776No Hit
GGTTAGTAGTTTTCTCGATTCAGCTGAAAATCCTTCGAGTTGAAGCAGAC14080.10964280430161115No Hit
GTGTTGAGAATGGAGCGATTTCTCTTGGGAATCCATGAATGTGTGGGATT14040.10933131906211795No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC13950.10863047727325821No Hit
GATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCCAATGATTGTC13740.10699517976591885No Hit
GAACAGGAACAGGATACACCATGGACACAGTCAACAGAACACATCAATAT13620.1060607240474392No Hit
ATGCACACACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTG13610.1059828527375659No Hit
GTTATATAGATTGTTCACGTTCGCATCATGCTCATCGAGTGTTTTTTGAT13540.10543775356845278No Hit
GGCCTCCATCATGCTGGAAATTCCAACTGGCCTCCGATATGAACTGCTAG13380.10419181261047993No Hit
TTCTTCTTTCTTAATCCTTCCAGACTCGAAGTCAATCCGTGCATCAATTC13360.10403606999073332No Hit
ATCTTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTTCCAGACTCGAA13350.10395819868086001No Hit
ATTCGGGCCCTAGACACCATGGCCTCCATCATGCTGGAAATTCCAACTGG13200.10279012903276045No Hit
CTATTCGAGAAATTCTTCCCTAGCAGTTCATATCGGAGGCCAGTTGGAAT13100.10201141593402742No Hit
ACAGTGCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGG13060.10169993069453422No Hit
CTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATCTTCAAGATCT12920.10060973235630796No Hit
GTCCTACATCGTCGAAAGATCATCAGCTGTAAATGGAACGTGTTACCCTG12910.10053186104643466No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTATTA100.007091271144.9988145
GCAGGTA17300.0116.515532
CAGGTAC18000.0112.790023
GGTACTG18450.0109.253055
TCCACCG203.8664375E-4108.7491145
AGTAGAA19100.0106.6738741
GTACTGA19950.0101.3980266
AGCGAAA2700.096.6771551
GCGAAAG3000.087.009442
AGCAGGT24100.083.639781
GGTCAAA1550.079.524761
CGAAAGC3300.079.099493
ACTGATC27150.078.507648
TAGAAAC26500.076.88573
AGAAACA26800.076.025044
TCTCCGC2150.074.185425145
TTTCGTT809.822543E-1172.4994145
GCGAATA300.00193593272.49939145
CCCACGC1200.072.49939145
AGGTACT29550.071.403694
Produced by FastQC (version 0.11.5)