FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2232_d04_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2232_d04_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences704636
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG45850.6506905693152208No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCC36100.5123212552296504No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG24520.3479810852695576No Hit
GTATAGGTGGGTGGGTGATGTATGCCCCACACGAAAAGAATGCTCTTTCC24340.3454265748556702No Hit
CTATTAGTAGAGGTCTTATTTTCTCAATTTTCTTTCTCGTCGATTCGTTG24270.34443315413915837No Hit
GTCCTACATCGTCGAAAGATCATCAGCTGTAAATGGAACGTGTTACCCTG22020.3125017739655652No Hit
CACCTATACCGAGCAAACAAATTTGTACATAAGAAACGACACAACAACAA20190.28653091809104275No Hit
GTTATATAGATTGTTCACGTTCGCATCATGCTCATCGAGTGTTTTTTGAT19510.2768805454163568No Hit
TCCATGCACTGATCATCACATTTATGGTATAGCTCGAAACAGCCTTTCCC14580.20691534352488378No Hit
GTTTTAGTACCGACCAATAATAATCAATTCTTCCCTGCAGACCATTGACA14170.201096736471029No Hit
GTACTAAAACCAGGCCAAACATTGCGAGTACGATCCAATGGGAATCTAAT13800.19584579839803815No Hit
CTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCA13010.18463433602597654No Hit
ATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTG12920.18335708081903282No Hit
GAATAATATAGTCGACAAGATGAACAAGCAATATGAAATAATTGATCATG12890.18293132908338489No Hit
ATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAA11640.16519167343138869No Hit
ATGTAGGACCATTCTCTTCCTCCCAACAGCAGGTCACAAGAAGGGTTGCC11570.1641982527148769No Hit
GTATGGACACGTTCTTTCAGGAGGGAGCCATGGAAGAATCCTGAAGACTG11330.16079223882969365No Hit
CACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGAACG10350.1468843487985286No Hit
CTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTGCATCGGC10270.14574901083680084No Hit
ATTGAAGGACTAGTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGAGG10240.14532325910115293No Hit
GCTCCACACAGAGCATAATGGAATGCTGTGTGCAACAAGCCTGGGACATC10050.14262683144204952No Hit
ATATTATTCACCTTGGATGTTATTTTATCAATTGCCTTTTGAGTTGAATC9850.1397884865377301No Hit
CCTGTAGAATGAACCTGAACATGCTCTGCTTGTTCCAGTGTAAGTCACAT9640.13680822438819476No Hit
TAACTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTGCATC9430.1338279622386594No Hit
CTATATAACAAGGTGAAGAGGGCACTGGGCTCCAATGCTATGGAAGATGG9090.12900277590131642No Hit
TCTCTATACTTTCTCCTATTATAGGTCCCGTTCCGAATTGTTTCCATGCA9080.12886085865610045No Hit
CTCCTGTAGAATGAACCTGAACATGCTCTGCTTGTTCCAGTGTAAGTCAC9010.12786743793958866No Hit
GTATAGCATGGTGGGTTCTTGGGACCATTGGATCTTTACAGTCTCCCAAT8750.12417758956397346No Hit
TGTCCATACCATGGAGCAATTAGATTCCCATTGGATCGTACTCGCAATGT8590.12190691364051794No Hit
ATATAGCGGTATCCCCTCCCCCTCATGGCTCGGGTCCTCAATGCTTAATT8510.1207715756787902No Hit
GGGTAAAAACCGCTCTTTTGAGTCAGCCATCTCATACTCCTGTAGAATGA8480.12034582394314228No Hit
GCCTATATTCATCTCCACAACTCGAAGGATTTTCAGCTGAATCGAGAAAA8440.1197781549622784No Hit
CAATATGATCAATAATAAGATTGATGACCAAATACAAGACGTATGGGCAT8430.11963623771706243No Hit
GTTCAAGACGCCCAATACACAAATAACAGGGGAAAGAGCATTCTTTTCGT8390.11906856873619853No Hit
AGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGT8370.1187847342457666No Hit
GTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGAGGAAGAGAATGGTC8250.11708172730317498No Hit
CAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTG8230.11679789281274303No Hit
GTCCCTAAGTGCTTGAACAATGAGTAGTAGTTTTCTCGATTCAGCTGAAA8050.11424338239885558No Hit
GTACTGTTTAAGCCACCTTTTTCAGTCTGACATTGCACTACACAATTACC7970.11310804443712782No Hit
ATTCTAGACACATGCACTATTGAAGGACTAGTCTATGGCAACCCTTCTTG7900.11211462372061602No Hit
GTCTTGAACAGGGTAAAAACCGCTCTTTTGAGTCAGCCATCTCATACTCC7840.11126312024932021No Hit
CTACTAATAGAGGGCACAGCCTCATTGAGTCCAGGGATGATGATGGGCAT7730.10970203055194455No Hit
GTACTAGCAATTCTGCATTATATGCCCATACGTCTTGTATTTGGTCATCA7670.10885052708064873No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA7560.10728943738327307No Hit
ATATAGAAGTATTGCATTTGACTCAAGGAACTTGCTGGGAGCAGATGTAC7440.10558643044068143No Hit
ATACAAGACGTATGGGCATATAATGCAGAATTGCTAGTACTACTTGAAAA7410.10516067870503351No Hit
AGTTAACACATATCAATGGATCATTAGGAATTGGGAGACTGTAAAGATCC7370.10459300972416966No Hit
GTGTTACCCTGGGAATGTAGAAAACCTAGAGGAACTCAGGACACTTTTTA7300.10359958900765785No Hit
TTCTACAGGAGTATGAGATGGCTGACTCAAAAGAGCGGTTTTTACCCTGT7110.10090316134855444No Hit
ATTATAGGTCCCGTTCCGAATTGTTTCCATGCACTGATCATCACATTTAT7100.10076124410333846No Hit
CTATTAACTCTTACATATTTGGGGCAGGTTCCAAATGCATATTTACTGAT7050.10005165787725862No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAATGT100.0070847995145.038471
CTACGAC100.007090816144.9973145
CCACGCG100.007090816144.9973145
GCAGGTA4650.0121.645172
GGTACTG4750.0119.084215
CAGGTAC5200.0108.7788543
GTACTGA5800.0101.276866
CTCCGAC259.386283E-486.99838145
GGCAAAC502.267916E-886.998387
GCTCTAT353.432051E-582.879131
GTTAAGT353.432051E-582.879132
CCACGAC353.4368873E-582.855606145
CCCACGC451.2326436E-680.55406145
GAGCCCC451.2326436E-680.55406145
GCGAAAG554.384492E-879.111892
AGGTACT7300.078.479724
CTTATAA755.638867E-1177.331894145
TGCCGTG406.662437E-572.49865145
GACAGTA6350.065.077537
TTTGGAC6850.060.344483
Produced by FastQC (version 0.11.5)