FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2232_d04_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2232_d04_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1536969
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCC65560.42655382118962715No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG62510.4067095692886454No Hit
CTATTAGTAGAGGTCTTATTTTCTCAATTTTCTTTCTCGTCGATTCGTTG53240.34639605613385827No Hit
GTTATATAGATTGTTCACGTTCGCATCATGCTCATCGAGTGTTTTTTGAT46730.304039964371435No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG41140.2676696797397996No Hit
GTCCTACATCGTCGAAAGATCATCAGCTGTAAATGGAACGTGTTACCCTG37810.24600366045118674No Hit
GTATAGGTGGGTGGGTGATGTATGCCCCACACGAAAAGAATGCTCTTTCC30330.19733644595304134No Hit
ATATTATTCACCTTGGATGTTATTTTATCAATTGCCTTTTGAGTTGAATC30230.1966858147431731No Hit
CACCTATACCGAGCAAACAAATTTGTACATAAGAAACGACACAACAACAA29450.1916108913062007No Hit
ATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAA27580.17944408768166437No Hit
GAATAATATAGTCGACAAGATGAACAAGCAATATGAAATAATTGATCATG26200.17046537698548248No Hit
GTTTTAGTACCGACCAATAATAATCAATTCTTCCCTGCAGACCATTGACA25700.1672122209361412No Hit
TCCATGCACTGATCATCACATTTATGGTATAGCTCGAAACAGCCTTTCCC25330.16480488545962865No Hit
GTACTAAAACCAGGCCAAACATTGCGAGTACGATCCAATGGGAATCTAAT25150.1636337492818658No Hit
ATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTG23470.15270314495607915No Hit
ATGTAGGACCATTCTCTTCCTCCCAACAGCAGGTCACAAGAAGGGTTGCC22660.14743303215614628No Hit
CACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGAACG20270.13188294624029503No Hit
ATATAGCGGTATCCCCTCCCCCTCATGGCTCGGGTCCTCAATGCTTAATT20090.13071181006253216No Hit
GTATGGACACGTTCTTTCAGGAGGGAGCCATGGAAGAATCCTGAAGACTG19850.12915029515884835No Hit
TAACTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTGCATC19620.12765384337615138No Hit
CTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCA19310.1256368866255598No Hit
CAATATGATCAATAATAAGATTGATGACCAAATACAAGACGTATGGGCAT19220.12505131853667836No Hit
CTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTGCATCGGC18350.11939082701082455No Hit
GCCTATATTCATCTCCACAACTCGAAGGATTTTCAGCTGAATCGAGAAAA18110.11782931210714075No Hit
GTACTAGCAATTCTGCATTATATGCCCATACGTCTTGTATTTGGTCATCA17230.11210375746030012No Hit
GTTCAAGACGCCCAATACACAAATAACAGGGGAAAGAGCATTCTTTTCGT17100.11125793688747138No Hit
ATTGAAGGACTAGTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGAGG17030.11080249504056361No Hit
GTGTTACCCTGGGAATGTAGAAAACCTAGAGGAACTCAGGACACTTTTTA16940.11021692695168217No Hit
CTATATAACAAGGTGAAGAGGGCACTGGGCTCCAATGCTATGGAAGATGG16920.11008680070970851No Hit
CCTGTAGAATGAACCTGAACATGCTCTGCTTGTTCCAGTGTAAGTCACAT16510.10741921274924869No Hit
GTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGAGGAAGAGAATGGTC16370.10650832905543313No Hit
ATTATATACAAATGTTGCATCTGCAAGATCCATTGGACATGGCCCAGAAC16110.10481668790977568No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT15800.10279973115918407No Hit
GTACTGTTTAAGCCACCTTTTTCAGTCTGACATTGCACTACACAATTACC15790.10273466803819725No Hit
TTGTTATATAGATTGTTCACGTTCGCATCATGCTCATCGAGTGTTTTTTG15720.10227922619128949No Hit
CTCCTGTAGAATGAACCTGAACATGCTCTGCTTGTTCCAGTGTAAGTCAC15590.10143340561846075No Hit
ATCATATTGAGTCTAGTTTCAACCTCACTGAATTCATGATCAATTATTTC15440.10045745880365836No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGGTA9450.0117.417352
GGTACTG10050.0109.6893165
GTATCCG203.866691E-4108.748344145
CCCACGC800.099.68598145
CAGGTAC11300.098.835953
GTACTGA11300.098.1973656
AGTAGAA5400.094.0075761
CTCCGAC451.2333148E-680.55433145
GACTTAA451.2333148E-680.55433145
CGAGACA451.2333148E-680.55433145
GCGAAAG1000.079.7747342
AGGTACT14500.079.0245064
CACGAGC702.8139766E-972.49889145
GCTAGGT406.664828E-572.49889145
CCACGAC702.8139766E-972.49889145
CGAAAGC1150.069.369333
TCTCCGC2950.066.35492145
TAGGACA350.003567083562.141907145
GTGGTCC350.003567083562.141907145
AACACTG3000.055.6023835
Produced by FastQC (version 0.11.5)