FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2232_d02_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2232_d02_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2317517
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTAGTAGAGGTCTTATTTTCTCAATTTTCTTTCTCGTCGATTCGTTG75910.3275488378294528No Hit
GTTATATAGATTGTTCACGTTCGCATCATGCTCATCGAGTGTTTTTTGAT41870.18066749887918837No Hit
GAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCGAGATA36000.15533866633988014No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT29950.12923314046887252No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG25710.11093769754439774No Hit
GTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACATCTTCGGCGTTGT23560.10166052719354378No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGCG3500.051.7940672
CGAAAGC8000.051.6645853
CACGAGC3800.043.88108145
TGATCCG6700.041.1207129
CTCCGAC1953.6379788E-1240.896973145
GTACTGA8800.036.256636
ACGCGTT1209.570034E-636.249588145
GCGAAAG11550.035.7849962
GTCATAG7850.035.1012042
CTATTAG30900.034.730521
GTATAGG13000.033.4669341
GGATAGC7350.032.5569765
TATTAGT32650.032.424832
GCAGGTA8900.031.7747862
TATACTA8150.031.1406635
TAGTAGA33250.030.53195
GTAATCG1902.0787957E-730.52597145
TCTCCGC7900.030.28447145
TGTAGCG1204.0553333E-430.2138585
TAGAGGT34600.028.9171078
Produced by FastQC (version 0.11.5)