FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2232_d02_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2232_d02_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1276080
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTATATAGATTGTTCACGTTCGCATCATGCTCATCGAGTGTTTTTTGAT25680.20124130148580027No Hit
CTATTAGTAGAGGTCTTATTTTCTCAATTTTCTTTCTCGTCGATTCGTTG14730.11543163438028964No Hit
GTATAGGTGGGTGGGTGATGTATGCCCCACACGAAAAGAATGCTCTTTCC14070.11025954485612188No Hit
GTCCTACATCGTCGAAAGATCATCAGCTGTAAATGGAACGTGTTACCCTG13830.10837878502915178No Hit
GAATAATATAGTCGACAAGATGAACAAGCAATATGAAATAATTGATCATG13610.10665475518776252No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC5800.045.0083663
GGTACTG4100.042.4469155
TGATCCG3950.040.3793459
GCGAAAG6750.038.673862
TATAGCG3900.037.18642
ACTATAC6550.033.212283
TCTAACG1751.00964826E-733.1425258
TAACTAT4850.032.892711
AGCGAAA8500.031.5646931
TATACTA5750.030.2664975
TAGATTG14550.029.3983337
GTCATAG5750.029.0053922
TTGTACG1753.7702284E-629.0053924
TTATATA17250.028.1646562
GTACTGA6500.027.88986
TATATAG16650.027.873053
TATACTG7400.027.4375325
CAGGTAC5350.027.1078453
AGGACGG2954.820322E-1027.0389255
GTTATAT17300.026.4066451
Produced by FastQC (version 0.11.5)