FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2231_d12_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2231_d12_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2642302
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC48630.18404406460730077No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG39440.1492637858957833No Hit
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC38270.1448358287584084No Hit
GTTGACATGGTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCCCAT36100.1366232928711404No Hit
GTTTTCTTCTTGTAATTGATTTCTAGTTCTATACCACTGTGGTTGGAAAA33710.1275781496588959No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA31320.11853300644665145No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT29860.11300752147180752No Hit
CTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTCATT28660.10846602697193583No Hit
GTCTTTTTCTTTAGGATTCAATCTTAGTAGGTTGTATGTTTCTGGAAATG28120.10642235444699358No Hit
GCATGGAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAAG27040.10233500939710903No Hit
GCAGTGCAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAA26610.10070764053465502No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGAT259.37907E-487.0219355
TCGACGG1056.913069E-848.33271145
GTTGACA25950.043.8734251
ACATGGT27850.041.1414265
TCTCCGC7000.038.320934145
GTATTAT25950.036.887211
TATACTG11000.034.9406245
GGTCCTA39800.034.7935839
TAGCGTA3450.033.6316645
GATAGCG4100.033.597126145
GACATGG34150.033.3392944
GCGAAAG5850.032.2303472
TGGTCCT43300.032.1492168
ATGGTCC44500.031.2816777
GCAGTGC19350.030.7306791
TATAATA16050.030.7242552
CTCCGAC2401.5843398E-930.207941145
GTGATCG2901.2732926E-1130.0007489
GTACTGC9200.029.95324
CATGGTC38300.029.9167216
Produced by FastQC (version 0.11.5)