FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2231_d12_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2231_d12_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1775597
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC37740.21254823025720362No Hit
GTTGACATGGTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCCCAT33240.18720464159378508No Hit
GCAGTGCAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAA28820.16231160561771618No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA27830.1567360161117641No Hit
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC26690.1503156403170314No Hit
ATCTATGATGAACACAAACATAAAAATGCTCACCCAAATATCTGCAAACC25470.14344471183494903No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG25450.14333207366311163No Hit
GCATGGAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAAG24490.13792544141491564No Hit
GTCTTTTTCTTTAGGATTCAATCTTAGTAGGTTGTATGTTTCTGGAAATG22930.1291396640115972No Hit
CTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTCATT22030.12407094627891352No Hit
AAGTTGGAGAACGTTCTAGGGGCACTTGAGAGGAGTATATTCCTTTTGCT21350.12024124843644138No Hit
ATCTTAGTAGGTTGTATGTTTCTGGAAATGTATCCTTTTCTTTTGGATTA21110.11888959037439238No Hit
TTACTGCCCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA20070.11303240543884677No Hit
GTTGACATGGTCCTATGATGTTGATCCTTTGTTTTGTGAATGCGGCACAT19950.11235657640782228No Hit
GTTTTCTTCTTGTAATTGATTTCTAGTTCTATACCACTGTGGTTGGAAAA19600.11038540840066749No Hit
GTTTTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTGGAATTGA18960.10678098690187018No Hit
CTTATATGGACTTGACAGATACACACAGAACCCTCCATCCAAAAGCAGCA18560.10452822346512186No Hit
AGTCTGTTCAGATGTCTTTTTCTTTAGGATTCAATCTTAGTAGGTTGTAT18350.103345522660829No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGACA20150.056.8545461
ACATGGT21250.053.230565
GACATGG26050.043.1438944
GGTCCTA32150.042.3958479
GTTACGT1401.4142643E-841.4328771
TGGTCCT33000.041.3049968
ATGGTCC33450.040.9660767
GCAGTGC15950.040.0041581
CAGTGCA16250.039.712942
TACTGCG750.001482734138.6717725
TCTCCGC5100.038.38202145
AGTGCAG17100.037.73893
CATGGTC30850.037.372286
GCTACTA6050.037.149489
ATCTATG15100.035.5334931
CACGAGC4100.035.365543145
AGCGAAA3700.035.2739331
TGACATG33300.034.8394363
AGTCCGC1501.1346274E-633.83304145
CTCCGAC1501.1346274E-633.83304145