FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2231_d10_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2231_d10_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1309103
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT31330.23932417846418502No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG23690.1809636063778022No Hit
GTATTGGGAGTCAGGATATGGTCATTGGAGAAGAATTTATTGTGTTTATT23090.17638031537625382No Hit
GTCTTTTTCTTTAGGATTCAATCTTAGTAGGTTGTATGTTTCTGGAAATG20540.15690132861967315No Hit
ATTTCATTTATTTAAGTTCTGATGTGTTCCTTCTACTAACTTTGGACTTT19470.1487277930002452No Hit
CTCCACCTTAGGTCATATGTAAGGCCACAAGACAAGTCAATAAATTCCAG18680.14269312651487315No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC17970.13726956549637423No Hit
GTTGACATGGTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCCCAT17550.13406126179529035No Hit
AGTCTGTTCAGATGTCTTTTTCTTTAGGATTCAATCTTAGTAGGTTGTAT17280.1319987808445936No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA17030.13008907626061508No Hit
GATATTCCCTGAAACCATTGATATGCAAAGGCTCTTAAGAATGTACTATG16580.1266516080094538No Hit
GTTTTCTTCTTGTAATTGATTTCTAGTTCTATACCACTGTGGTTGGAAAA16280.1243599625086796No Hit
ATCTATGATGAACACAAACATAAAAATGCTCACCCAAATATCTGCAAACC15510.11847807239002583No Hit
AGATATTCCCTGAAACCATTGATATGCAAAGGCTCTTAAGAATGTACTAT15000.11458227503870971No Hit
CCACCTTAGGTCATATGTAAGGCCACAAGACAAGTCAATAAATTCCAGAA14080.10755456216966885No Hit
ATCTTAGTAGGTTGTATGTTTCTGGAAATGTATCCTTTTCTTTTGGATTA13980.10679068033607746No Hit
GCATGGAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAAG13600.10388792936843014No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTT400.00605150454.374542145
TTACGTG953.1377567E-853.4267232
CCGACTA3000.045.9215434
ACATGGT11850.040.995915
GCGAAAG2057.2759576E-1238.9065672
CGATGTT750.001482779938.6707735
TCTCCGC3450.037.825768145
GTTGACA13050.037.781791
GTTACGT1354.968824E-737.5965841
GTGATCG1001.3914407E-436.2510769
ATGCCGG2201.6370905E-1136.24969145
GCTACTA3450.035.72579
CTCCGAC1051.85412E-434.52352145
CCACCGA3800.034.345751
ATCTATG8450.034.323171
GTACTGC8400.033.6642884
GCAGTGC10150.033.5749931
AGCGAAA2404.3655746E-1133.2326931
GGTCCTA17900.033.213289
AGTCCGC1751.00968464E-733.14258145
Produced by FastQC (version 0.11.5)