FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2231_d08_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2231_d08_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1676474
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT35720.21306623305819236No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG25200.1503154835684896No Hit
ATTTCATTTATTTAAGTTCTGATGTGTTCCTTCTACTAACTTTGGACTTT25030.14930145054441643No Hit
GTATTGGGAGTCAGGATATGGTCATTGGAGAAGAATTTATTGTGTTTATT20970.12508395596949312No Hit
GTCTTTTTCTTTAGGATTCAATCTTAGTAGGTTGTATGTTTCTGGAAATG18920.11285591067919931No Hit
GATATTCCCTGAAACCATTGATATGCAAAGGCTCTTAAGAATGTACTATG17990.10730855354750507No Hit
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC17300.10319277244979642No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG17180.1024769844328036No Hit
GTTTTCTTCTTGTAATTGATTTCTAGTTCTATACCACTGTGGTTGGAAAA17140.10223838842713935No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC16840.10044891838465733No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGACT1900.049.612274
AGACCGC1650.048.332954145
CAAGCGG951.9197996E-645.7959443
CACGAGC3500.045.57107145
GTCGTCG657.338291E-444.61504145
TATACTG8250.043.066695
GTTACGT2450.041.433191
GGTGCGA1401.4151738E-841.429488
GTGCGAT1755.2750693E-1141.4282469
CGAAGCG700.001056886641.4282469
TTACGTG2500.040.605742
GGGTTAC2300.037.8303031
AAGACGA2550.036.9660072
AGACGAC2600.036.2551233
TGCGTTA3000.036.2507978
GCGTTAA3050.035.6554579
GACTTAA7800.035.324451
CGAAAGC3300.035.1564832
CCTTATA6200.035.08562
TAACCTA4350.035.0049485
Produced by FastQC (version 0.11.5)