FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2231_d08_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2231_d08_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1579104
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG21780.13792631770928324No Hit
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC20210.1279839706567775No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT19650.12443765578454619No Hit
GTCTTTTTCTTTAGGATTCAATCTTAGTAGGTTGTATGTTTCTGGAAATG19510.12355107706648835No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC19030.12051137860457577No Hit
GTTTTCTTCTTGTAATTGATTTCTAGTTCTATACCACTGTGGTTGGAAAA16720.10588282975662147No Hit
GATATTCCCTGAAACCATTGATATGCAAAGGCTCTTAAGAATGTACTATG16530.10467961578211442No Hit
AGTCTGTTCAGATGTCTTTTTCTTTAGGATTCAATCTTAGTAGGTTGTAT15990.10125995501246277No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGTG2150.050.5920452
GTTACGT2200.049.440661
ACCGTAC855.3372692E-542.654691
GTTGACA13600.039.4555851
ACATGGT13550.039.0672655
GCGTGCG1502.600791E-838.6674589
GGTTATA5450.038.584881
GACGACT1354.965623E-737.600514
GGTGCGA1354.971789E-737.5945558
ATCTATG7950.036.4845161
GCGAAAG3500.035.2217032
ACGTGTT3100.035.088034
CGAAAGC3550.034.725623
TATACTG8350.033.8694085
GTATTAT15100.033.615281
GGGTTAC1956.9849193E-933.4675251
AGCGAAA3750.032.8725471
CCGAGGT4450.032.591134
CCGACTA2900.032.5068444
CCGTACA1153.15902E-431.5283742
Produced by FastQC (version 0.11.5)