FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2231_d06_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2231_d06_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1761173
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG62970.35754579476292225No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTTAATCC62600.3554449222194526No Hit
GTATAGGTGGGTGGGTGATGTATGCCCCACACGAAAAGAATGCTCTTTCC59820.3396599879739242No Hit
GTTATATAGATTGTTCACGTTCGCATCATGCTCATCGAGTGTTTTTTGAT50680.2877627581163236No Hit
CACCTATACCGAGCAAACAAATTTGTACATAAGAAACGACACAACAACAA50220.28515086252173977No Hit
GTCCTACATCGTCGAAAGATCATCAGCTGTAAATGGAACGTGTTACCCTG49500.2810626781128259No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG43600.2475622780953376No Hit
ATATTATTCACCTTGGATGTTATTTTATCAATTGCCTTTTGAGTTGAATC42690.2423952672451826No Hit
CACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGAACG40500.22996037300140304No Hit
GTACTAAAACCAGGCCAAACATTGCGAGTACGATCCAATGGGAATCTAAT40250.22854086452608574No Hit
GTTTTAGTACCGACCAATAATAATCAATTCTTCCCTGCAGACCATTGACA38210.21695767536749655No Hit
GAATAATATAGTCGACAAGATGAACAAGCAATATGAAATAATTGATCATG33300.18907852891226473No Hit
TAACTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTGCATC32600.1851039051813763No Hit
CTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTGCATCGGC32500.18453610179124935No Hit
TCCATGCACTGATCATCACATTTATGGTATAGCTCGAAACAGCCTTTCCC32070.18209454721370358No Hit
ATGTAGGACCATTCTCTTCCTCCCAACAGCAGGTCACAAGAAGGGTTGCC31800.1805614780603609No Hit
CTATATAACAAGGTGAAGAGGGCACTGGGCTCCAATGCTATGGAAGATGG29150.1655146882219975No Hit
ATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAA28770.16335703533951518No Hit
CTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCA28230.16029089703282984No Hit
ATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTG27580.15660017499700482No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT26600.15103570177376102No Hit
ATTGAAGGACTAGTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGAGG25970.1474585404159614No Hit
CTTCAATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTA25160.14285933295593334No Hit
TTCCAACACTGTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTCC25130.14268899193889528No Hit
GTATGGACACGTTCTTTCAGGAGGGAGCCATGGAAGAATCCTGAAGACTG24240.13763554176676568No Hit
GCTCCACACAGAGCATAATGGAATGCTGTGTGCAACAAGCCTGGGACATC24190.1373516400717022No Hit
GTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGAGGAAGAGAATGGTC23720.13468296413810568No Hit
CCTGTAGAATGAACCTGAACATGCTCTGCTTGTTCCAGTGTAAGTCACAT23710.134626183799093No Hit
GGGTAAAAACCGCTCTTTTGAGTCAGCCATCTCATACTCCTGTAGAATGA23700.1345694034600803No Hit
TTCTTAATCCTTCCAGACTCGAAGTCAATCCGTGCATCAATTCGGGCCCT23020.13070834040721724No Hit
GTTCAAGACGCCCAATACACAAATAACAGGGGAAAGAGCATTCTTTTCGT22650.12860746786374763No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA22560.12809644481263338No Hit
ATATAGCGGTATCCCCTCCCCCTCATGGCTCGGGTCCTCAATGCTTAATT22330.12679049701534148No Hit
GCCTATATTCATCTCCACAACTCGAAGGATTTTCAGCTGAATCGAGAAAA21970.12474640481088457No Hit
ATTATATACAAATGTTGCATCTGCAAGATCCATTGGACATGGCCCAGAAC21930.1245192834548338No Hit
GCTTTAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATAT21610.12270231260642765No Hit
CTCCTGTAGAATGAACCTGAACATGCTCTGCTTGTTCCAGTGTAAGTCAC21540.1223048502333388No Hit
TGTCCATACCATGGAGCAATTAGATTCCCATTGGATCGTACTCGCAATGT21440.12173704684321188No Hit
TCTCTATACTTTCTCCTATTATAGGTCCCGTTCCGAATTGTTTCCATGCA21340.12116924345308495No Hit
CATTTATGGTATAGCTCGAAACAGCCTTTCCCATCTTCCATAGCATTGGA21050.11952261362171689No Hit
TTATATGCCCATACGTCTTGTATTTGGTCATCAATCTTATTATTGATCAT20090.11407170107649844No Hit
TTTCTTAAAAGTGCCAGCGCAAAATGCAATAAGTACCACATTCCCTTATA20050.11384457972044768No Hit
GTACTAGCAATTCTGCATTATATGCCCATACGTCTTGTATTTGGTCATCA20040.11378779938143499No Hit
GTGTTACCCTGGGAATGTAGAAAACCTAGAGGAACTCAGGACACTTTTTA19910.11304965497426998No Hit
CTAATAACTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTG19890.1129360942962446No Hit
CTATTGAAGGACTAGTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGA19870.11282253361821921No Hit
GTTTTTACCCTGTTCAAGACGCCCAATACACAAATAACAGGGGAAAGAGC19820.11253863192315576No Hit
CCATTGGACATGGCCCAGAACAGGAAGGCAGCAAACCCCATTGCAAGCAC19650.11157336615993999No Hit
TTCTACAGGAGTATGAGATGGCTGACTCAAAAGAGCGGTTTTTACCCTGT19430.11032419870166076No Hit
ATCATATTGAGTCTAGTTTCAACCTCACTGAATTCATGATCAATTATTTC19330.10975639531153386No Hit
GTCTTGAACAGGGTAAAAACCGCTCTTTTGAGTCAGCCATCTCATACTCC19010.10793942446312771No Hit
GTACTGTTTAAGCCACCTTTTTCAGTCTGACATTGCACTACACAATTACC18910.10737162107300079No Hit
ATTTGGAGCCATAGCTGGATTCATAGAAGGAGGTTGGCCAGGACTAGTCG18670.10600889293669617No Hit
CCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAG18550.10532752886854387No Hit
CAATATGATCAATAATAAGATTGATGACCAAATACAAGACGTATGGGCAT18490.10498684683446771No Hit
ATTCTAGACACATGCACTATTGAAGGACTAGTCTATGGCAACCCTTCTTG18440.10470294513940424No Hit
GTCCCTAAGTGCTTGAACAATGAGTAGTAGTTTTCTCGATTCAGCTGAAA18350.10419192208829002No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG18030.10237495123988387No Hit
GTACATTGCCATTCCACAATATCAGTAAATATGCATTTGGAACCTGCCCC17980.1020910495448204No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACTG7400.0110.7275165
GCAGGTA7750.0106.662562
CAGGTAC8850.092.585733
GTACTGA9000.091.0426256
GAGTATT353.4381283E-582.856544145
AGTAGAA9350.081.428081
TAGCGCG451.2333312E-680.55498145
AGGTACT10700.077.933274
GGTCAAA2550.076.775051
GACTTAA851.8189894E-1276.764145145
AAATATA2650.073.8799745
CGTAAGC300.001936043772.49947145
AACACTG4950.070.31455
GCGAAAG1800.068.483392
GACAGTA10550.068.0346157
CGCAATT808.090865E-963.43704145
GCTTATA350.003564649762.1529962
CCACGAC606.8080335E-660.416225145
TTTAAAC6250.058.0094643
CGAAAGC2200.056.0318643
Produced by FastQC (version 0.11.5)