FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2231_d04_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2231_d04_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1864495
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTAATTGATATGCAAAAAACCCCGGCCTTATTGACCGGGGTTTTTCT114950.6165208273553965No Hit
GGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACG88480.4745520905124444No Hit
CTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGATGTTTTGGCGGA83440.4475206423186976No Hit
GTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGC81980.439690103754636No Hit
GGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGAT77260.414374937985889No Hit
CATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGCCTA74170.3978020858194846No Hit
GTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACATCTTCGGCGTTGT73100.39206326646089157No Hit
GTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGATGTTTTGGC72030.3863244471022985No Hit
AATCTAATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACATCT71770.3849299676319861No Hit
GGATAAGACGCGCCAGCGTCGCATCCGGTATTAATTGATATGCAAAAAAC64060.34357828795464723No Hit
CTCTAAGTCTCAGGGAGAACTCATCTCGGGGCAAGTTTCGTGCTTAGATG59340.3182631221859002No Hit
CGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTG59170.3173513471476191No Hit
GACTTAGAGTCTCCTGAAGGAACGTTGAAGACGACGACGTTGATAGGCCG54910.2945033373648092No Hit
TCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGG54790.2938597314554343No Hit
TCATTAACCCGCTTGTCGCCGGTTAATCTAATCTGTATCAGGCTGAAAAT48580.260553125645282No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGA46700.2504699663984082No Hit
CCCTGAGACTTAGAGTCTCCTGAAGGAACGTTGAAGACGACGACGTTGAT45730.24526748529762754No Hit
GATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATA45500.24403390730465888No Hit
GATTAGATTAACCGGCGACAAGCGGGTTAATGAAACAGAATTTGCCTGGC44510.23872415855231577No Hit
TCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGT43080.23105452146559793No Hit
ATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGT42690.22896280226012944No Hit
AATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACATCTTCGGC42300.22687108305466092No Hit
TCACTGGTGTTCGGGTTGTCATGCCAATGGCACTGCCCGGTAGCTAAATG41710.22370668733356752No Hit
GTCTCAGGGAGAACTCATCTCGGGGCAAGTTTCGTGCTTAGATGCTTTCA41300.22150770047653653No Hit
GCCTTATTGACCGGGGTTTTTCTAATTTGATGCCTGGCAGTTCCCTACTC41270.2213467989991928No Hit
GATAAGTGCTGAAAGCATCTAAGCACGAAACTTGCCCCGAGATGAGTTCT40690.21823603710388068No Hit
GTGTAAGCGCAGCGATGCGTTGAGCTAACCGGTACTAATGAACCGTGAGG39720.21303355600310003No Hit
GTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGA39570.2122290486163814No Hit
GTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTT36440.19544166114685207No Hit
CATCACTGGTGTTCGGGTTGTCATGCCAATGGCACTGCCCGGTAGCTAAA36080.19351084341872732No Hit
GTCTCACGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGAACA35410.189917377091384No Hit
ACATACGCTTATACGTTTAGACATCCAGATAGCTGAAGATAACCAGAAAA35390.18981010943982152No Hit
TAGTACGAGAGGACCGGAGTGGACGCATCACTGGTGTTCGGGTTGTCATG34670.18594847398357195No Hit
TTGTAAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCATC34620.18568030485466575No Hit
GGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCG34350.18423219155857218No Hit
ATCTAATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACATCTT33120.1776352309874792No Hit
ACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTG33040.17720616038122924No Hit
TTTCAGCACTTATCTCTTCCGCATTTAGCTACCGGGCAGTGCCATTGGCA32850.17618711769138562No Hit
CTCCTGAAGGAACGTTGAAGACGACGACGTTGATAGGCCGGGTGTGTAAG32140.1723791160609173No Hit
GCCCGGTAGCTAAATGCGGAAGAGATAAGTGCTGAAAGCATCTAAGCACG32040.17184277780310486No Hit
GTGCTGAAAGCATCTAAGCACGAAACTTGCCCCGAGATGAGTTCTCCCTG31340.1680884099984178No Hit
CTAATAACCATTTTCTCTCCTTTTCGTCATTCTTATATTCTAACGTAGCC30560.1639049715874808No Hit
GCAAAAAACCCCGGCCTTATTGACCGGGGTTTTTCTAATTTGATGCCTGG30230.16213505533669975No Hit
CCAGTGATGCGTCCACTCCGGTCCTCTCGTACTAGGAGCAGCCCCCCTCA30100.16143781560154358No Hit
GTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTAGTA29980.16079420969216865No Hit
CTACATACGCTTATACGTTTAGACATCCAGATAGCTGAAGATAACCAGAA29080.15596716537185673No Hit
GGGTCAGGTGGGACCACCGCGCTAAGGCCGCCAGGCAAATTCTGTTTCAT28820.15457268590154438No Hit
GAGTGGACGCATCACTGGTGTTCGGGTTGTCATGCCAATGGCACTGCCCG28670.15376817851482574No Hit
TCCATATGCTGCCCACTTAACAACATAAACCCAACCGGCTGATCCCGCTC28350.15205189608982594No Hit
TCCTGGGGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGT28260.15156919165779476No Hit
CTTACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGGAG27850.14937020480076377No Hit
CCTAGTACGAGAGGACCGGAGTGGACGCATCACTGGTGTTCGGGTTGTCA27710.14861933123982632No Hit
CTTCAGGAGACTCTAAGTCTCAGGGAGAACTCATCTCGGGGCAAGTTTCG27420.14706395029217026No Hit
TATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTAGTACGAGA27250.14615217525388913No Hit
GGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGCCTATCAACGTCGT27030.14497223108670176No Hit
GCCTGATACAGATTAGATTAACCGGCGACAAGCGGGTTAATGAAACAGAA26720.14330958248748318No Hit
TCCTTCAGGAGACTCTAAGTCTCAGGGAGAACTCATCTCGGGGCAAGTTT26670.14304141335857698No Hit
CAATGGCACTGCCCGGTAGCTAAATGCGGAAGAGATAAGTGCTGAAAGCA26060.13976974998592112No Hit
GTCATGCCAATGGCACTGCCCGGTAGCTAAATGCGGAAGAGATAAGTGCT25850.138643439644515No Hit
ACCTTACAACGCCGAAGATGTTTTGGCGGATGAGAGAAGATTTTCAGCCT25700.13783893225779636No Hit
ACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATG25640.1375171293031089No Hit
GTGTTCGGGTTGTCATGCCAATGGCACTGCCCGGTAGCTAAATGCGGAAG25280.1355863115749841No Hit
GTACGAGAGGACCGGAGTGGACGCATCACTGGTGTTCGGGTTGTCATGCC24940.1337627614984218No Hit
GAGCTAACCGGTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAG24850.13328005706639065No Hit
GTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGCCTATCAAC24440.13108107020935963No Hit
GTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGAT24410.1309201687320159No Hit
TTGATAGGCCGGGTGTGTAAGCGCAGCGATGCGTTGAGCTAACCGGTACT24340.1305447319515472No Hit
CGCTTATACGTTTAGACATCCAGATAGCTGAAGATAACCAGAAAATTTAC24190.12974022456482853No Hit
GCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGGAGACTCTAAGTCTC24010.12877481570076615No Hit
AAAGTAGGCTGGATAAGACGCGCCAGCGTCGCATCCGGTATTAATTGATA23890.12813120979139123No Hit
GAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAG23890.12813120979139123No Hit
CGCTAAGGCCGCCAGGCAAATTCTGTTTCATTAACCCGCTTGTCGCCGGT23650.12684399797264137No Hit
GGTTAAGGATAAGCACCTTTAGCGGACGAATTTCCTGACCAGACGCGCGA22790.12223148895545444No Hit
GGTGGGACCACCGCGCTAAGGCCGCCAGGCAAATTCTGTTTCATTAACCC22750.12201695365232944No Hit
GTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCC22720.12185605217498571No Hit
GAGGGGGGCTGCTCCTAGTACGAGAGGACCGGAGTGGACGCATCACTGGT22640.12142698156873577No Hit
TATCTCTTCCGCATTTAGCTACCGGGCAGTGCCATTGGCATGACAACCCG22640.12142698156873577No Hit
GTTTGGCACCTCGATGTCGGCTCATCACATCCTGGGGCTGAAGTAGGTCC22510.12072974183357961No Hit
GGTTAATCTAATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAAC22290.11954979766639225No Hit
AGCAAAAGTAGGCTGGATAAGACGCGCCAGCGTCGCATCCGGTATTAATT22090.11847712115076736No Hit
GTTGTAAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCAT22000.11799441671873617No Hit
GTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC21880.11735081080936124No Hit
ATACAGATTAGATTAACCGGCGACAAGCGGGTTAATGAAACAGAATTTGC21870.11729717698358No Hit
GATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCAC21820.1170290078546738No Hit
GCACTTATCTCTTCCGCATTTAGCTACCGGGCAGTGCCATTGGCATGACA21580.11574179603592394No Hit
AACCGGTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGATGTT21120.11327464004998672No Hit
GCCATACCCTTGGGACCTACTTCAGCCCCAGGATGTGATGAGCCGACATC20980.11252376648904933No Hit
GCTCAACGCATCGCTGCGCTTACACACCCGGCCTATCAACGTCGTCGTCT20870.11193379440545563No Hit
GAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAG20780.11145108997342443No Hit
ATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAA20740.11123655467029947No Hit
AAGCAGGACGGATAAGCAAAAGTAGGCTGGATAAGACGCGCCAGCGTCGC20540.1101638781546746No Hit
CCTGAAGGAACGTTGAAGACGACGACGTTGATAGGCCGGGTGTGTAAGCG20520.1100566105031121No Hit
CTTATCTCTTCCGCATTTAGCTACCGGGCAGTGCCATTGGCATGACAACC20130.10796489129764358No Hit
CTCGTACTAGGAGCAGCCCCCCTCAGTTCTCCAGCGCCCACGGCAGATAG19860.10651677800155002No Hit
AATGAACCGTGAGGCTTAACCTTACAACGCCGAAGATGTTTTGGCGGATG19710.10571227061483136No Hit
CTTATATTCTAACGTAGCCTTTTCCTTGAAACTTTCTCACCTTCAACATG19500.10458596027342523No Hit
GGAGTGGACGCATCACTGGTGTTCGGGTTGTCATGCCAATGGCACTGCCC19260.1032987484546754No Hit
TTCTTATATTCTAACGTAGCCTTTTCCTTGAAACTTTCTCACCTTCAACA19240.10319148080311291No Hit
CCTTCAGGAGACTCTAAGTCTCAGGGAGAACTCATCTCGGGGCAAGTTTC19220.10308421315155042No Hit
GCATATGGATGCGCTGTTTATCGATCCTGATGTGCGCGGCTGCGGCGTGG18980.10179700133280058No Hit
GTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCT18800.10083159246873818No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCATAG17300.074.6029362
CATAGTG17500.073.750334
GCGAAAG8250.055.3692472
GACGGAT6600.054.9268657
AGTGATC23850.054.11167
CAGGACG7050.053.48064
CGGTCCG701.6966158E-551.785408145
TGATCCG25350.051.7663659
GGACGGA7050.051.425036
AGGACGG7450.049.6359065
GTTAAGG7200.047.3311842
GTTGACA8000.047.1285131
ATAGTGA28800.046.827665
GTGATCC28200.046.793018
TAAGACG25550.046.540924
CGCGCCA28300.046.3702249
CGAAAGC9950.045.9091763
ACATGGT8150.045.3726965
TAAGGAT7700.045.1993874
ATAAGAC27000.044.578593
Produced by FastQC (version 0.11.5)