FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2231_d02_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2231_d02_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2481649
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC48390.1949913142430698No Hit
CTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTCATT28770.11593097976385863No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGAGC7000.047.64233145
TACTGCG2400.042.302285
GCGTTAA2700.037.5929379
TCTCCGC9800.031.071089145
GTATTAT20100.030.30551
CTCCGAC2451.9754225E-929.591513145
GTTGACA24650.028.8301811
ACTGCGT4050.028.6491646
ACATGGT23900.028.5218015
ACCGTCG1050.007710272427.61938
ATCTATG12450.026.7933331
GTGATCG3255.0931703E-1126.7694769
ATAGTCG1907.1481973E-626.7166923
CGCTATA1658.410292E-526.3697222
TGCGTTA3850.026.363888
GATAGCG4400.026.363348145
GAATAAC19200.026.0607011
TATAGCG1701.03027865E-425.5941432
TATAGTG25450.022.7955065
GTTCTGA23950.022.1032221
Produced by FastQC (version 0.11.5)