FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2231_d02_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2231_d02_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1595182
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC27320.17126572391112738No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGAC1600.058.90593145
CGCTATA858.922725E-751.181012
TCTCCGC5200.032.067135145
TATAGCG1153.1612936E-431.5245322
CACGAGC5700.030.526152145
ATCGTCG4600.029.946438
GCGAAAG5150.028.1578371
ACATCGT5750.027.742466
TACGTTG1050.007705291327.622365
GAATAAC12700.026.2621691
TACATCG5250.026.2412435
TACCGAC4150.026.2055028
GTACCGA3900.026.0263167
ACGTAGC2259.113646E-725.77764145
GTCCTAT20200.025.125991
TATACAT7300.024.8317475
TATACTG9750.023.7977285
TCCTATG19050.023.597892
GTACTGC3803.6379788E-1022.8967674
TATTAGT10800.022.8260972
Produced by FastQC (version 0.11.5)