FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2230_d12_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2230_d12_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1686794
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG6500.047.9708752
CGAAAGC6600.047.2440453
AGCGAAA6800.045.854511
CGCGCCA1707.8271114E-834.1182179
CACGAGC3200.033.983936145
GACGCGC1102.4370395E-432.954127
ACGCGCC1855.8184833E-627.4328928
TACGCCG1658.410793E-526.3687695
GTGATCG1658.4209765E-526.3640799
TCTCCGC5650.025.663385145
TAAGCGC2101.553548E-524.1713714
TATACTG8900.023.6281935
GTAATCG1550.001809137323.386795145
GTATATA10150.022.8615911
GTATTAT18100.021.2334161
CTCCGAC2505.978207E-520.299738145
GTGTAGG9750.020.0808321
GCACCGT4702.3646862E-1020.0570956
TACACCG2205.8979314E-419.7765755
GTATAAG4853.601599E-1019.4367711
Produced by FastQC (version 0.11.5)