FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2230_d12_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2230_d12_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1564021
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC17400.11125170314209337No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA5450.042.5815931
GCGAAAG5800.041.263712
CGAAAGC6050.038.359853
TCTCCGC7700.034.836987145
GTCCTAT15700.027.2534071
TCCTATG16750.024.6798422
TATGATG17100.024.5988185
GTATAAG5450.021.2907961
CTATGAT19500.021.1993524
ATGATGT19400.020.9347926
GTCTAGG4851.6370905E-1120.934121
TGATCCG1750.003652600320.721175
ACCGTTC2556.9565955E-519.9034828
CTAGTAC6000.019.339763
GAATAAC9900.019.0461241
CAAGTCG2307.962301E-418.9138879
GTATACT3101.4634432E-518.7158974
TATACTA4654.483809E-918.7158975
GCACCGT3953.1145646E-718.360536
GTATTAT15550.018.1887441
Produced by FastQC (version 0.11.5)