FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2230_d10_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2230_d10_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1793854
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA6250.040.608571
GCGAAAG7350.035.51772
CTCCGAC3200.031.718372145
CGAAAGC8900.029.3320333
ACCGTAC1000.00606946429.006121
TTATTCG1855.8200512E-627.432104145
TCTCCGC6950.025.035673145
GTCCTAT12650.021.7832551
ATTATAC8600.021.0800293
GTATATA10300.020.4169311
TATACTG8450.019.7378925
CTAGACG3001.104311E-519.3374163
GTGTAGG12150.018.5018481
GTATTAT17100.017.8107781
TATATAC11450.017.7333
TCCTATG12850.017.4939652
GTTATAC6303.6379788E-1117.2655493
GTATAAT8200.016.8023261
TTATACC13050.016.6701854
GTATAAG6104.2564352E-1016.6428571
Produced by FastQC (version 0.11.5)