FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2230_d10_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2230_d10_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1318428
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA4950.042.4855461
GCGAAAG5700.038.1675952
TAGTACG800.002034309636.2592164
CCGTACA950.00472195930.5340772
GTATAAG3500.029.0073761
CGAAAGC7900.027.5386473
GTAGTCG1358.0720865E-426.858683
TTTACGA1550.001806119223.3930452
CACGAGC3452.9667717E-923.115683145
TAGTACC6650.021.8042689
CTGGTCG2053.6675748E-421.2192739
TATAAGA7350.020.7195532
GTATTAG4752.728484E-1019.8471531
GAGTCGA2205.894625E-419.7777566
TCTCCGC4853.619789E-1019.432772145
TCTCGTT2256.869787E-419.333115145
GTATAGG2701.0773975E-418.8010771
TCTATAC15850.018.758713
GTATAAA12750.018.2007061
GTTATAT4404.8567017E-818.1296081
Produced by FastQC (version 0.11.5)