FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2230_d08_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2230_d08_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1874269
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATAG450.009640790548.33289145
GTTACGT2003.2526077E-729.0043741
CACGAGC4150.027.951551145
TATACTG9250.025.869465
AGCGAAA5100.025.5920961
TACACCG3709.094947E-1225.4774975
GTGCGAT2301.1040665E-625.217169
ACACCGT4201.8189894E-1224.170966
GTGCGCT1500.001494839924.1664439
GGTGCGA2151.8666136E-523.6050628
GTTATGT11400.023.5342521
GTATAAG6000.022.9617981
GCGAAAG5800.022.5039962
GTACCGT1952.6127853E-422.3116533
GTTGACA15050.022.1628111
GATAGCG4607.2759576E-1222.065016145
TTACGTG2455.110073E-520.7179642
ACATGGT16200.020.1424665
TACCGTC2205.898948E-419.7762374
CGAAAGC6700.019.4810713
Produced by FastQC (version 0.11.5)