FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2230_d08_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2230_d08_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1703483
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG5700.045.7984922
AGCGAAA5950.043.8741871
CGTCGAG907.485038E-540.2774358
CGAAAGC6750.039.748573
TAAGGCG850.002739876734.124374
GCGTCGA1255.1556015E-428.9997527
ACCGTTC3005.875336E-1026.5831098
TCTCCGC8850.025.395264145
CTCCGAC3102.4207111E-823.386898145
GTATATA9800.021.4583071
GATATAT7800.020.4527451
TAACGTG2205.89787E-419.7766235
TATAATA11700.019.2131862
TAGTACC8850.018.8416489
TACTCCG2000.00790931718.128574
ATGTCGG2050.00912941417.682775145
GCTATAT5351.3351382E-917.6202931
GTAATAC6653.6379788E-1217.4470443
TCTATAC20850.017.0417253
ATATAGG6404.5474735E-1116.9955351
Produced by FastQC (version 0.11.5)