FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2230_d06_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2230_d06_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2132673
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG5050.061.739332
AGCGAAA5450.057.2080041
CGAAAGC8050.038.7308853
TTATTCG1502.6020643E-838.6663145
TTACGGA2752.1282176E-1029.0031264
TCTCCGC9900.027.095707145
AGACCGC3306.184564E-1126.36339145
CTCCGAC3357.4578566E-1125.969904145
GTATTAG9950.025.5052641
CAGCGCG1450.001226463125.0015249
GTATTAT26050.023.10231
TACTGCG3055.338061E-721.3967535
CTTACGG3757.765266E-921.2689593
TACGGAG4151.0913936E-920.96715
TATAATA20100.020.9226552
TAGTACC7350.020.7155489
GTATTAA25900.019.8766611
TATACGG2959.557769E-619.664065
ACGTCCG2959.565514E-619.6622169
TAGTACT8900.019.552674
Produced by FastQC (version 0.11.5)