FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2230_d06_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2230_d06_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1535721
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC22460.14625052336980482No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA4700.038.5710531
ACCGTAC1001.3902642E-436.2567861
GCGAAAG6550.028.7840162
GAATAAC8100.028.6473391
TCTCCGC5950.028.024982145
ACGATCG1606.8308575E-527.18728145
GTATTAG7050.026.7425981
TATTAGG5900.025.8099172
CGAAAGC8150.024.9126423
CTCCGAC3052.0367224E-823.7703145
GTATTCG2151.8638782E-523.6090743
GTGATCG2202.2299499E-523.0679959
TCGCCAT3803.6379788E-1022.89455145
CTAATAC8300.022.7150963
GATAGCG2252.6529042E-522.555372145
GTCCTAT12800.022.093981
TATACAG9900.021.9738125
GTATAAC4006.89397E-1021.7540721
CCACGAC3055.3444273E-721.39327145
TATCGAG5750.020.1776921
Produced by FastQC (version 0.11.5)