FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2230_d04_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2230_d04_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1888383
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG5600.038.8421862
CGAAAGC6350.034.2545243
AGCGAAA6700.032.4642521
CTCCGAC2401.5843398E-930.208189145
ATAGTCG1358.0843613E-426.8524349
TACACCG2051.2900429E-524.7586025
TCTCCGC8050.021.614803145
TAGTACC9200.020.4895769
GTATTAT22050.020.3864691
GTATTAC6850.020.1104581
GTATAGC4051.8835635E-819.6923081
CTAATAC9000.019.3347763
GCACCGT6151.8189894E-1218.8636976
AGACCGT3602.6166563E-618.1268336
ACGTCCG2801.4266942E-418.1253939
TATAACG3252.202737E-517.847965
TATAATA16800.016.8316152
TACACTG11250.016.757255
AGCACCG6959.094947E-1216.6923375
TATTATA15050.016.3799612
Produced by FastQC (version 0.11.5)