FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2230_d04_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2230_d04_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1544223
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG4550.036.655172
AGCGAAA4600.036.2567441
ATCCGCG800.0020370436.2497029
CGAAAGC5950.028.0304243
GTGATCG2001.0660267E-525.3747929
GTATTAT14750.023.1059931
TCTCCGC7450.022.382366145
GAACGAT1750.003655677720.7181386
TATACGA1750.003655677720.7181385
ACGATCG2205.905689E-419.772564145
GTCCTAT10850.019.3814851
ATACCGA1900.005882981219.0824996
GAGTGCG2701.0794537E-418.796757
GTATATA10450.018.7355421
TATACTG7000.018.6463245
TAGTACC8200.018.5705284
CTATACT6001.8189894E-1118.1283724
CTCCGAC2851.633843E-417.80687145
TAACGTG2050.00911881817.6862165
GTACCTA7800.017.6635426
Produced by FastQC (version 0.11.5)