FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2230_d02_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2230_d02_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1889911
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC25710.1360381520611288No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG4800.036.2555052
AGCGAAA5450.031.9306091
ACTCGCG1450.00122689724.999826145
CGAAAGC7200.024.1703363
GTGATCG3001.7093043E-824.16659
GATAGCG1801.5210558E-424.166498145
CCACGAC4154.5474735E-1122.710688145
TATACTG12000.021.7533045
TCTCCGC5750.021.434633145
GTTGACA12600.021.292351
CTCCGAC2755.215028E-621.090763145
CACGAGC2205.9063063E-419.77259145
CGTACAC2307.9574E-418.9159153
GTACAGG10000.018.8523641
GGTCCTA18500.018.810689
CCGTCGT2701.0798732E-418.796165145
GTATTAT17550.018.5920731
ACGCTAA3953.1222044E-718.3567311
TACTGCG2000.00791651818.1258327
CTGTGCG2450.001217525617.754989
Produced by FastQC (version 0.11.5)