FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2230_d02_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2230_d02_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1550126
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG4400.054.3914832
AGCGAAA4600.050.450071
ATATCGA907.466733E-540.2938846
CGAAAGC5850.039.670143
GATAGCG1854.1854946E-935.26958145
AAGGCGC3800.032.43358145
TATATCG950.00471959630.5375375
GTACCGC1050.007694709627.630096
GTAGACG1100.00965891526.3716283
TCTCCGC8050.023.41569145
ACCGTAC1550.001808123723.3889039
GTGATCG2552.7068636E-622.7468559
TAGTATA5450.021.2908554
TATAATA11950.021.2407462
GTAGTAC2404.354689E-521.1522433
GCGAATG4801.4551915E-1121.14542145
GGACTAC4851.6370905E-1120.9341771
TAAGACC8450.020.5979584
GTATAAT7950.020.0689751
GTATTAT14850.020.0229021
Produced by FastQC (version 0.11.5)