FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2044_d08_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2044_d08_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1662694
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC16670.1002589772982882No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGCC3050.045.1635868
CGAGACG1002.7353854E-643.49967145
AACGCGC1252.7205533E-740.5996867
CGCGCCA3350.038.9549269
TCTCCGC7450.033.086994145
GACGCGC2601.10958354E-1030.6728427
TTATTCG1204.0593746E-430.208103145
TGATCCG2651.382432E-1030.094119
CGAAAGC5250.029.0041393
AGCGAAA5500.029.0041391
GCGAAAG5550.028.742842
ATCGCGG2603.7234713E-927.884401145
CACGAGC4850.026.907011145
TATAATA11900.025.5918882
GTATAAT7200.025.1772041
GTATTAT16200.025.0653081
GTATTAA30900.022.2928261
ATTATAC7850.022.1687683
TATACGG1650.002599423821.9728325
TATTAAT19700.021.7162952
Produced by FastQC (version 0.11.5)