FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_2044_d08_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_2044_d08_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1685406
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCGC1201.9742583E-742.2923857
GCGAAAG5800.038.7598572
AGCGAAA5950.037.7815971
CGCGCCA4150.036.6862839
ACGCGCC4550.033.462118
TGATCCG3250.031.2303739
TCTCCGC7000.031.071035145
GTGATCG2601.10958354E-1030.6726889
CGAAAGC7800.029.7511563
ACTCGCC1555.5019904E-528.0649958
GACGCGC3650.027.8086917
TACACCG3251.4824764E-924.5447035
AAACGCG2303.137504E-522.0708316
GAATAAC11300.021.8190611
TAAGACG5750.020.1790454
ATAAGAC9150.019.8137843
GTATTAT13850.019.3725831
GTGTTCC20800.019.1704319
CACGAGC7200.019.131702145
TATACTG8800.018.9536845
Produced by FastQC (version 0.11.5)