FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_1805_d12_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_1805_d12_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2803606
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACGG2750.063.271793145
GCGAAAG10100.033.7455522
AGCGAAA10650.032.002251
CGAAAGC12450.027.9583763
TCTCCGC13550.026.217325145
GCACCGT12600.024.7487626
TCGCGTT2651.3783756E-724.622276145
AGCACCG13550.023.0131975
TTATTCG1952.6166366E-422.307364145
ACCGTGT15200.021.4657968
TATACTG15700.020.785515
CCACGAC7350.020.713982145
CACCGTG16500.020.6523157
AATCGGT1800.004302232520.144346
GACTCGC4351.9681465E-919.999704145
ACCCGCG3701.5708065E-719.5964018
TGTCGTT3701.5725891E-719.594305145
CACGAGC6300.019.563204145
TAGTACT6400.019.2623394
TATACTA10000.018.8547675
Produced by FastQC (version 0.11.5)