FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_1805_d10_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_1805_d10_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2791470
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA12300.050.1089361
GCGAAAG14750.043.2613222
CGAAAGC15450.041.3012663
TCTCCGC11500.029.62998145
CACGAGC11550.023.85245145
ACCGATA1550.001809458623.386738145
GTATATA15700.020.3214361
AGACCGC7200.020.13858145
TACACCG7400.018.6182485
TCCGCTA1950.006840624418.5911229
GTATTAT22800.018.4456751
GAGCGAA6750.018.2619231
GTATAGA19250.018.0805591
GTATTAA26300.017.3694551
GTATAAA28050.017.0613141
GTATACG5151.5919795E-816.8956681
CTATACT9150.016.6423684
AGGCGCG7001.0913936E-1116.572667
ATTATAC13700.016.4077973
GCGCGGA4451.0887507E-616.2933449
Produced by FastQC (version 0.11.5)