FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_1805_d08_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_1805_d08_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1790336
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC9100.033.4686553
GCGAAAG8950.032.409132
CACGAGC4550.031.867788145
AGCGAAA9500.030.5328081
CGTAATT1050.00770940727.6195228
TACACCG3151.033186E-925.3228455
TACTACG2051.2888799E-524.7613645
GCCGTCG1500.001494827624.166407145
TGAGCGC8150.024.018147145
TATACTG10150.021.4331285
GTCTAGG6600.020.875651
TACGAGC1750.003659480520.7146428
TATGCGG3601.17472155E-720.1431735
GCGATCG3601.1774864E-720.138672145
GCACCGT9800.019.9792966
CCCTACG1900.005882435519.0830042
AGCACCG10350.018.9170655
TCTCCGC7300.018.86966145
ACCGTTC3502.0005446E-618.6431778
AGTACCG3151.6844906E-518.4130138
Produced by FastQC (version 0.11.5)