FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_1805_d06_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_1805_d06_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1817227
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACGG1406.60415E-736.2565965
CCGTACG900.003625772232.228092
CGATAGG1402.7444863E-531.0770843
TGATCCG3350.030.2990199
ACCGCGT1555.5022283E-528.0649918
CGGTAGA1958.747265E-626.0303781
CTATGCG3151.033186E-925.322074
CCGATAG1450.001225528225.004552
CCGACTA3801.2732926E-1124.8071464
CGAAAGC9200.023.6456072
TACGGGG1902.1938761E-422.8951237
ACCGTAC1600.002173529522.6603741
GTTACGT2303.1394324E-522.0692331
TATACAG10900.021.2882795
GTATAGG3757.75799E-921.2705381
GATCGAT6850.021.168247
ATACGCT11150.020.8109633
AGTCCGC2455.1173756E-520.714064145
TATGCTG16500.020.6552725
TATACTG8450.020.5954655
Produced by FastQC (version 0.11.5)