FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_1805_d04_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_1805_d04_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1085802
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC13410.1235031801378152No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGGC1750.049.713512145
ACCGGCA657.32578E-444.6290743
CGGCACG657.32578E-444.6290745
GCGAAAG3850.043.3249781
TAGCGCG700.001056761841.42793145
CGAAAGC4400.036.2611242
ATACCGT800.00203362736.2611246
AGCGAAA3200.031.7284831
AGTAGCG1204.0509834E-430.2176062
CACGAGC2354.2989996E-827.765524145
TACACCG1050.00769720327.6275235
GTTGACA7500.024.1740841
CGGAGTA1851.8270031E-423.5207314
TCTCGTT2552.707804E-622.744745145
TCTCCGC4205.2750693E-1122.440126145
ATGCCGG4205.2750693E-1122.440126145
GCACCGT8100.022.383416
CCTATAT4557.2759576E-1222.3145391
AGCACCG8000.021.7566765
GGTCCTA13100.021.5836359
Produced by FastQC (version 0.11.5)