FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_1805_d02_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_1805_d02_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1380123
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC20840.1510010339658132No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGAC1351.9099389E-1048.33296145
AGACGGC3300.048.33296145
GCGAAAG3500.041.4357572
GTGATCG1905.4296834E-934.3418359
TACATCG3250.033.461277145
ATGCCGG3150.032.221973145
CGAAAGC4850.031.3971963
TGCACCG1204.0547154E-430.2135725
TCTCGTT3650.029.794289145
TATACTG10000.029.0050285
ACTCCGC1804.7019457E-628.1942258
GTCCTAT13100.026.5694941
TAGTCCG1100.0096646626.3682085
AGCGAAA5550.026.1306571
TCTCCGC4800.025.676884145
GTAGGCG1851.8312536E-423.513339
TTACGGA1550.001807011323.3911534
CACGAGC2903.288169E-722.499825145
GTATATA10100.021.538391
TATATAC10350.021.0181373
Produced by FastQC (version 0.11.5)