FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_1803_d12_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_1803_d12_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences672496
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG2450.038.472132
AACCGCT800.002036172436.249897
AGCGAAA2800.033.6631131
CGAAAGC3050.033.2810633
GTGATCG1453.4887853E-529.9999089
CGTAACA1050.007704003727.6210162
CCCTACG1100.00966601726.3655152
CCTATAA3657.2759576E-1225.8237571
CGAGAAG1751.2545203E-424.8589133
GTATAGG2451.9093477E-623.6751561
GCTAATG3503.4960976E-922.785645145
GTATAAT3554.116373E-922.466391
GTACTAA2353.7048158E-521.5972841
CTATAGA4201.2641976E-920.7157631
GTACTGC2505.9674974E-520.3014476
GTATATA5453.6379788E-1219.9555511
TAGTATC1850.005043111719.5959914
TAGTACC2608.072486E-519.519179
GGATAGT2307.9544215E-418.9143926
CTATAAC2701.0779118E-418.7976382
Produced by FastQC (version 0.11.5)