FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_1803_d10_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_1803_d10_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2051415
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG7600.035.304222
ACTACGC1402.7437336E-531.0786171
CGAAAGC9800.028.8587173
AGCGAAA9800.027.3787821
TATACTG13700.026.9959535
CACGAGC6200.025.725487145
TAGTACC11000.021.0957894
GTATAGA23700.020.80651
CTACGCA2104.309531E-420.7190782
GTATAAT11450.019.6333621
GTATTAT23100.018.8355261
TACTACG2000.00790828518.1291942
GTTACGT3252.2004242E-517.8502831
TAGACAG14250.017.8115715
TACGCAC2851.6311803E-417.8111383
ACGCACT2500.001391911917.4040264
CGCACTG4307.5920616E-716.8647775
TTACGTG3453.674488E-516.8154832
GTATACT9950.016.762674
CCCTACG4358.569714E-716.6705232
Produced by FastQC (version 0.11.5)