FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_1803_d08_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_1803_d08_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2240103
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGCG1700.055.44071145
GCGAAAG9500.031.2947982
TCTCCGC8200.030.060722145
ATCGCGG2701.72804E-1029.536787145
TAATACG1504.396009E-529.0049364
CGAAAGC10450.027.7559193
AGCGAAA10350.027.3234861
CACGAGC5200.026.49016145
GGTGCGA1851.8314816E-423.5138388
GTCGAAG2552.705574E-622.7489683
TTACCGC1650.002599288521.9734361
TATACTG14900.021.8996985
AGTCGAA2003.1011325E-421.7536982
CTAGTAC4358.367351E-1121.6703533
CTCCGAC2353.7120666E-521.595562145
TACGAGG5101.8189894E-1221.3238267
GTGCGAT2455.1170915E-520.7145739
GTGATCG3551.01597834E-720.4228179
GTTTACG2608.0724625E-519.522551
CTACGCA1900.005884332619.0821932
Produced by FastQC (version 0.11.5)