FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_1803_d06_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_1803_d06_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1469131
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGCGG604.945948E-448.33313145
GCGAAAG7000.041.432632
AGCGAAA7150.039.5493241
CGAAAGC8300.034.943183
CTCCGAC1956.9976522E-933.461395145
GCGTAAA950.004725747330.5293031
GTTACGT1204.0566377E-430.211291
TGATCCG1050.00770976827.618939
CCGACTA2756.7630026E-926.3662194
TATACTG13000.025.6563575
CTATGCG1550.001808935923.3869979
TCTCCGC5750.022.695557145
GGTCGTT2903.288369E-722.4999057
GTAACGG1900.005885845519.0808141
TCGCCAT3451.7432158E-618.912964145
CACCGTT2701.0796426E-418.7962177
GAGCGAA3501.9985018E-618.6446841
GTATACG1950.006838185718.5915621
GTGTACA8800.018.1267741
GTGAACG2000.0079133318.1267744
Produced by FastQC (version 0.11.5)