FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_1803_d04_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_1803_d04_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1242019
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG5050.038.7697752
AGCGAAA5350.035.2403681
TCGTCGA1255.1548675E-428.9996939
CGAAAGC7400.026.457753
GGTAGTG6800.025.5889977
TATACTG10300.025.3446395
GACGCGG1500.001494568724.16641145
ACGTCCG1952.6154247E-422.3074579
GTACTAG3706.6393113E-921.5581671
TAGTGCA8450.021.4494789
ATCGTCG1700.003093399621.3233058
GTATAGG3757.750714E-921.2707231
AGGTAGT9150.020.6050226
TACATCG1800.004302893820.1427335
ATGCCGG2908.252882E-619.99979145
TGCCGTG4402.2646418E-919.772518145
TAAGGTA9350.019.3887254
GTATATA7900.019.2758271
TATAACG2659.3383154E-519.1545965
GTCCTAT10700.018.9755821
Produced by FastQC (version 0.11.5)