FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_1803_d02_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_1803_d02_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1726538
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACATCGTCGAAAGATCATCAGCTGTAAATGGAACGTGTTACCCTG72720.42118968710795823No Hit
GTATAGGTGGGTGGGTGATGTATGCCCCACACGAAAAGAATGCTCTTTCC70960.41099587729896475No Hit
CACCTATACCGAGCAAACAAATTTGTACATAAGAAACGACACAACAACAA64780.3752017042196581No Hit
GTACTAAAACCAGGCCAAACATTGCGAGTACGATCCAATGGGAATCTAAT50290.291276531417206No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG47860.2772021235559252No Hit
CTATTAGTAGAGGTCTTATTTTCTCAATTTTCTTTCTCGTCGATTCGTTG45380.26283811882507074No Hit
GTTTTAGTACCGACCAATAATAATCAATTCTTCCCTGCAGACCATTGACA42450.2458677422680532No Hit
TCCATGCACTGATCATCACATTTATGGTATAGCTCGAAACAGCCTTTCCC40410.2340521899894471No Hit
ATTGAAGGACTAGTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGAGG39920.23121414066762505No Hit
GTATGGACACGTTCTTTCAGGAGGGAGCCATGGAAGAATCCTGAAGACTG34470.19964808188409405No Hit
ATGTAGGACCATTCTCTTCCTCCCAACAGCAGGTCACAAGAAGGGTTGCC32030.18551575464889855No Hit
CCTGTAGAATGAACCTGAACATGCTCTGCTTGTTCCAGTGTAAGTCACAT30080.17422147673552507No Hit
ATACAAGACGTATGGGCATATAATGCAGAATTGCTAGTACTACTTGAAAA29890.1731210086311451No Hit
GTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGAGGAAGAGAATGGTC29250.16941416870060202No Hit
TGCTAGTACTACTTGAAAATCAAAAAACACTCGATGAGCATGATGCGAAC28950.16767658748315994No Hit
ATTCTAGACACATGCACTATTGAAGGACTAGTCTATGGCAACCCTTCTTG28370.16431726379610528No Hit
TGTCCATACCATGGAGCAATTAGATTCCCATTGGATCGTACTCGCAATGT27820.16113169823079482No Hit
GGGTAAAAACCGCTCTTTTGAGTCAGCCATCTCATACTCCTGTAGAATGA27580.15974163325684115No Hit
GTACTGTTTAAGCCACCTTTTTCAGTCTGACATTGCACTACACAATTACC27090.1569035839350191No Hit
GTGTTACCCTGGGAATGTAGAAAACCTAGAGGAACTCAGGACACTTTTTA27070.15678774518718963No Hit
CTATATAACAAGGTGAAGAGGGCACTGGGCTCCAATGCTATGGAAGATGG26920.15591895457846858No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG26660.15441305085668544No Hit
GTTATATAGATTGTTCACGTTCGCATCATGCTCATCGAGTGTTTTTTGAT26610.1541234539871118No Hit
CTCCTGTAGAATGAACCTGAACATGCTCTGCTTGTTCCAGTGTAAGTCAC25850.14972158156959187No Hit
CACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGAACG25580.148157758473894No Hit
CTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTGCATCGGC25440.14734688723908768No Hit
GTCTTGAACAGGGTAAAAACCGCTCTTTTGAGTCAGCCATCTCATACTCC24530.14207622421284677No Hit
GTTCAAGACGCCCAATACACAAATAACAGGGGAAAGAGCATTCTTTTCGT23920.13854314240404786No Hit
TTCTACAGGAGTATGAGATGGCTGACTCAAAAGAGCGGTTTTTACCCTGT23620.1368055611866058No Hit
GTTTAAGCCACCTTTTTCAGTCTGACATTGCACTACACAATTACCACCTT23280.13483630247350478No Hit
GGCATATAATGCAGAATTGCTAGTACTACTTGAAAATCAAAAAACACTCG22850.13234576939517115No Hit
GCTCCACACAGAGCATAATGGAATGCTGTGTGCAACAAGCCTGGGACATC22790.13199825315168273No Hit
CTATTGAAGGACTAGTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGA22400.12973939756900804No Hit
TCTCTATACTTTCTCCTATTATAGGTCCCGTTCCGAATTGTTTCCATGCA22320.12927604257769015No Hit
TTCTAGACACATGCACTATTGAAGGACTAGTCTATGGCAACCCTTCTTGT22290.12910228445594593No Hit
ATCTCATACTCCTGTAGAATGAACCTGAACATGCTCTGCTTGTTCCAGTG22260.12892852633420174No Hit
TGTATGCCCCACACGAAAAGAATGCTCTTTCCCCTGTTATTTGTGTATTG21960.12719094511675966No Hit
GTTTTTACCCTGTTCAAGACGCCCAATACACAAATAACAGGGGAAAGAGC21690.1256271220210618No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCC21620.12522168640365863No Hit
AGTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGAGGAAGAGAATGGT21590.12504792828191444No Hit
GCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATAT21020.12174652396877451No Hit
CTATTAACTCTTACATATTTGGGGCAGGTTCCAAATGCATATTTACTGAT20950.12134108835137136No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTTAATCC20860.12081981398613874No Hit
GTACATAATGAAGGGGGTTTACATAAATACAGCTTTGCTCAATGCATCTT20760.12024062024699136No Hit
ATCCAATGGGAATCTAATTGCTCCATGGTATGGACACGTTCTTTCAGGAG20270.11740257092516931No Hit
TAACTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTGCATC20060.11618626407295987No Hit
ATATTGTGGAATGGCAATGTACTGTTTAAGCCACCTTTTTCAGTCTGACA19950.11554915095989779No Hit
CTCATACTCCTGTAGAATGAACCTGAACATGCTCTGCTTGTTCCAGTGTA19820.11479619909900622No Hit
ACGTATGGGCATATAATGCAGAATTGCTAGTACTACTTGAAAATCAAAAA19460.11271110163807573No Hit
GTGCATGGAAACAATTCGGAACGGGACCTATAATAGGAGAAAGTATAGAG19170.1110314397945484No Hit
GCCTATATTCATCTCCACAACTCGAAGGATTTTCAGCTGAATCGAGAAAA18960.10981513294233895No Hit
ATTATAGGTCCCGTTCCGAATTGTTTCCATGCACTGATCATCACATTTAT18850.10917801982927686No Hit
ACACAATTACCACCTTTTAAATCAGTCTTCAGGATTCTTCCATGGCTCCC18760.10865674546404423No Hit
ACCAAATACAAGACGTATGGGCATATAATGCAGAATTGCTAGTACTACTT18520.10726668049009058No Hit
CTCTTACATATTTGGGGCAGGTTCCAAATGCATATTTACTGATATTGTGG17790.10303856619431487No Hit
ACCTAGAGGAACTCAGGACACTTTTTAGTTCCGCTAGTTCCTACCAAAGA17680.10240145308125279No Hit
GCTTTAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATAT17470.10118514622904332No Hit
CTACTAATAGAGGGCACAGCCTCATTGAGTCCAGGGATGATGATGGGCAT17420.10089554935946964No Hit
ATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTG17360.10054803311598122No Hit
TGTCAATGGTCTGCAGGGAAGAATTGATTATTATTGGTCGGTACTAAAAC17330.10037427499423703No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGGTA7350.089.776362
CAGGTAC8050.081.0689543
GGTACTG8350.079.897735
GTACTGA9800.068.818086
AGGTACT10250.064.376214
TAACTAT5200.055.7782141
GCGAAAG1206.002665E-1154.3837552
CTCCGAC1206.002665E-1154.374306145
GGATAGC17600.053.1508455
GACAGTA8400.050.0632327
AACTATA5800.050.008052
ACTATAC6300.049.4920923
TATGGAT20400.045.852972
CCCACGC2850.045.788887145
TTTGGAC10050.044.733573
CAATCCA8150.043.5985035
CTATTAG19450.043.2460371
TATACTA7500.042.5401842
CACGAGC2400.042.291126145
TATTAGT20200.041.9993362
Produced by FastQC (version 0.11.5)