FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_1802_d12_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_1802_d12_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1394206
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA8800.040.3734131
CGAAAGC9550.037.9619643
GCGAAAG9800.037.7334182
TACATCG800.002035876336.2536775
CTCCGAC1551.4656271E-632.741734145
GTATTAG4800.030.2113951
ACCGTTC1952.607885E-729.7444748
CTCGTAA1050.00770566327.621851
ACCGTCG2202.229109E-523.0688658
ATAGACT7250.023.0023334
TATAACG3203.3844117E-822.6585485
GACCGTC1600.002175655222.656117
AGGTGCG1650.00259983821.9719265
TGATCCG2053.664931E-421.2216645
TATAATA15800.021.1097352
GGACCGT1750.00365729420.7163876
TGCACCG1800.00430538120.140935
GATGCGC1800.00430716920.1394868
GACGGAT1800.004308063520.1387657
CGCCGAG1800.004308063520.1387657
Produced by FastQC (version 0.11.5)