FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_1802_d10_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_1802_d10_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1993914
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT1854.1763997E-935.2786671
CCGACTA3001.8189894E-1129.0069084
GCGAAAG8550.027.1409662
AGCGAAA8550.027.1409661
CGAAAGC8600.026.983173
CTCCGAC2351.3316949E-624.680538145
TATACTG14000.019.165285
ATGCCGG6550.018.816557145
GTCTAAC4654.491085E-918.7141341
GTCGAAG1950.006833486318.5941723
AGTCGAA2000.007907918.1293162
CGTATAC2851.6310661E-417.8112583
CACGAGC8150.017.791187145
CTACTGA20800.017.43166
GATATAT11650.017.4290431
CGAGATT2550.001589207917.0628874
GTATAGA19450.016.7777731
TATAATA21650.016.4126832
ACCGTTC4007.1436953E-616.3131128
CTACGCA2700.002328774216.1149484
Produced by FastQC (version 0.11.5)