FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_1802_d08_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_1802_d08_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1818900
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA7600.031.487641
GCGAAAG8100.029.5439592
ATAACCG1000.006068795529.0067985
CGAAAGC8450.027.4620573
TCTCCGC6700.027.051884145
CCGCGCG1851.8312903E-423.5138539
TACCGTC2953.869245E-722.1238294
TAACGAC2359.189546E-418.5149773
ATGCGCC2000.00791910218.124763145
TACACCG2450.00121553317.7592645
GTTAGCC5852.3464963E-1017.3544943
ATGCCGG5051.2512828E-817.227499145
TATACTG9950.016.7627225
ATTATAC11400.016.5389633
CTTAGAC10150.015.7179693
TCCGCAC4301.4154604E-515.1779753
TATAACG3355.592114E-415.1528045
CCACGAC3406.269272E-414.926275145
GTATAGA13950.014.5553821
AGTCCGG3000.004695828514.5037986
Produced by FastQC (version 0.11.5)