FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_1802_d06_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_1802_d06_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2311821
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT3150.032.232781
ACTACGC2705.456968E-1232.232781
AGCGAAA11000.029.0095021
GCGAAAG11700.027.275072
TCGCACC2808.174538E-925.9030234
GTTGACA15850.025.1659741
CGAAAGC12800.024.931663
TAATACG2502.2736021E-623.2091084
TACACCG3905.020411E-1022.3164525
TAGTCGG3406.435948E-821.324988
CTACTGA27300.021.2537616
CCGACTA6500.021.2006284
CTCGCAC3106.2277286E-721.056653
GCGCTTA1750.003659636220.7147989
CTACGCA4251.4661055E-920.4781822
ACATGGT19650.020.3005875
GTATTAG12650.020.065861
TATAATA28700.019.9638482
TATAGTA10000.019.5822622
TATACTG12250.019.538285
Produced by FastQC (version 0.11.5)