FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_1802_d04_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_1802_d04_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58412
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC750.12839827432719306No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATCCG100.0070738974145.05
CTAACCC350.003549715362.1428579
ATACACC350.003549715362.1428574
CCCACCC400.006022508354.3754
GATATAT400.006022508354.3751
ACATGGT400.006022508354.3755
TATAGTA400.006022508354.3755
GTATTAT701.6762317E-551.7857131
TTAGAAC700.00104848241.428573
GTAAGAG800.002020980236.259
GTGGTTG1102.4083127E-432.9545489
TATTATA900.003600540332.222222
CTGTTCA1050.007650134627.619059
GGGGGGG69350.010.03605145
TGGGGGG2100.00639471746.9047623140-144
AAGGGGG2500.004804676.3799996125-129
Produced by FastQC (version 0.11.5)