FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_1802_d02_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_1802_d02_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3354101
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC51330.1530365364668506No Hit
TATGCAAAGGCTCTTAAGAATGTACTATGAACAATTACACCCCAACAAGC40810.12167194726694278No Hit
GCAGTGCAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAA35310.1052741107080556No Hit
AGTCTGTTCAGATGTCTTTTTCTTTAGGATTCAATCTTAGTAGGTTGTAT34430.10265045685863365No Hit
GCATGGAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAAG34310.10229268587916704No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACGG2400.045.311977145
GTTGACA28600.043.8641971
GACTCGC3100.042.09629145
CTCCGAC2950.041.77918145
TATAGAC10850.037.427292
TATCGTC800.002035177536.257692
ACATGGT35100.035.741735
TCTCCGC12300.034.77602145
GGTCCTA41350.034.541149
GACATGG36900.034.1942444
GTGATCG5450.033.2574929
GCGATTA1751.0105032E-733.142475145
TTGACAT40400.031.0523782
CATGGTC41150.030.6635536
ATGGTCC47000.030.543577
GCGAAAG7450.030.1741872
GCAGTGC25400.029.6913381
TGGTCCT49400.029.3527648
ATCTATG26250.029.0061531
GACGTCC8700.026.666359145
Produced by FastQC (version 0.11.5)