FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_1801_d12_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_1801_d12_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1600793
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA7700.035.7854351
GCGAAAG7800.035.326652
TATAGCG850.002740141534.123572
CGAAAGC8850.031.95473
GTTACGT2651.382432E-1030.0995661
TTACGTG2603.7161954E-927.8894562
GTCTACG1050.007703281527.6238441
CACGAGC3409.094947E-1125.587881145
GATAGCG2502.2809163E-623.199678145
TATGCGA1600.002173522322.6601834
CCGACTA2353.7060057E-521.5994954
GTTGACA11450.021.5321241
TATACTG12200.021.3971585
ACGGTAG2455.10963E-520.7178845
ACCGTTC3208.4707244E-720.3922568
TACGGGG1800.004306189720.14057
GTGATCG2205.902311E-419.7743079
TGCGATG2608.0704085E-519.522625
TAAGCGC3001.104546E-519.336694
GTATTAT19600.018.8680731
Produced by FastQC (version 0.11.5)