FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_1801_d10_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_1801_d10_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2433537
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCT30890.12693458122888618No Hit
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC28090.11542869494073851No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGAGC6900.036.775143145
TAGCGCG1656.0232196E-835.151306145
GCGAAAG10650.033.361412
GATCCGC1352.1414371E-532.2266656
CTCCGAC2051.1401426E-831.82908145
AGCGAAA10650.031.3188721
GTTACGT2303.4873665E-828.3734761
CGAAAGC12800.027.7577343
ACGACTC2808.196366E-925.8964275
CCACGAC4300.025.290546145
TATACTG13000.024.5418455
GACGACT3152.8650902E-823.0190474
AGATCCG1600.002174297322.6593745
GAATCGC3203.385685E-822.6593741
CCGACTA3554.125468E-922.4678864
CTATGCG6900.022.068264
GTATAAT6950.021.9094961
GCCTATA4659.094947E-1221.8309671
TTAGGTA10300.021.8233994
TAGTACC12300.021.8088139
Produced by FastQC (version 0.11.5)