FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_1801_d08_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_1801_d08_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1927375
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCT29910.1551851611647967No Hit
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC26680.13842661651209548No Hit
GTGTAGCATTTGACATATAGAAAAGCACTTAGGGAAAATCATTGTGTGAC19930.103404890070692No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCGC800.002035367536.256223
ACGACTC2302.7284841E-1134.6807635
CACGAGC4250.034.117306145
GTTACGT1956.9885573E-933.467281
TTACGTG1956.9885573E-933.467282
AGCGAAA7450.033.0929261
CGACTCC2257.912604E-1032.228596
GGTGCGA1352.1429925E-532.2227368
GACGACT2301.0022632E-931.5271474
GTTGACA9950.031.3370341
TACACCG1703.0089905E-629.8588395
CCGACTA3450.029.4253394
GCGAAAG8150.029.3608672
TAATCGT1306.484544E-427.885069
CTCCGAC1306.485528E-427.884336145
CGAAAGC8650.027.6637063
ACGAACC5200.026.4956175
GCAGTGC8800.026.368161
GTGCGAT1658.4216386E-526.3640569
CAAGCGG1450.001225629525.004293
Produced by FastQC (version 0.11.5)